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Review
. 2022 Nov 14;11(11):1343.
doi: 10.3390/pathogens11111343.

High Throughput Sequencing for Clinical Tuberculosis: An Overview

Tara E Ness  1   2 , Andrew DiNardo  2 , Maha R Farhat  3
Affiliations
Review

High Throughput Sequencing for Clinical Tuberculosis: An Overview

Tara E Ness et al. Pathogens. .

Abstract

High throughput sequencing (HTS) can identify the presence of Mycobacterium tuberculosis DNA in a clinical sample while also providing information on drug susceptibility. Multiple studies have provided a context for exploring the clinical application of HTS for TB diagnosis. The workflow challenges, strengths and limitations of the various sequencing platforms, and tools used for analysis are presented to provide a framework for further innovations in the field.

Keywords: Mycobacterium tuberculosis; high throughput sequencing; next generation sequencing; sequencing; tuberculosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Workflow for high throughput sequencing of tuberculosis.
See this image and copyright information in PMC

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