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. 2022 Nov 18:15:6703-6709.
doi: 10.2147/IDR.S389959. eCollection 2022.

Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing

Omar B Ahmed  1
Affiliations

Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing

Omar B Ahmed. Infect Drug Resist. .

Abstract

Background: Multidrug-resistant Pseudomonas aeruginosa has become a hazard to public health, making medical treatment challenging and ineffective. Whole-genome sequencing for antibiotic susceptibility testing offers a powerful replacement for conventional microbiological methods.

Objective: The present study evaluated the presence of antibiotic resistance genes in selected clinical strains of P. aeruginosa using whole-genome sequencing for antibiotic susceptibility testing.

Results: Whole-genome sequencing of P. aeruginosa susceptible to common antibiotics showed the presence of 4 antibiotic resistance gene types, fosA, catB7, blaPAO, and blaOXA-50. Whole genome sequencing of resistant or multidrug-resistant P. aeruginosa showed the presence of multiple ARGs, such as sul1, aac(3)-Ic, blaPAO, blaGES-1, blaGES-5 aph (3')-XV, blaOXA-50, aacA4, catB7, aph(3')-IIb, aadA6, fosA, tet(G), cmlA1, aac(6')Ib-cr, and rmtF.

Conclusion: The acquisition of antibiotic resistance genes was found to depend on the resistance of Pseudomonas to antibiotics. The strain with the highest resistance to antibiotics had the highest acquisition of antibiotic resistance genes. MDR-P. aeruginosa produces antibiotic resistance genes against aminoglycoside, β-lactam, fluoroquinolones, sulfonamides, phenicol, and fosfomycin antibiotics.

Keywords: Pseudomonas aeruginosa; antibiotic resistance; genes; sequencing; whole genome.

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Conflict of interest statement

The author reports no conflicts of interest in this work.

Figures

Figure 1
Figure 1
PCR results showing the P. aeruginosa Opr L gene, M: marker (100 bp), Line 1 and 3: positive control, Lines 2: negative control, lines 4,5,6: strains of P. aeruginosa.
See this image and copyright information in PMC

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