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. 2022 Sep 30:45:108644.
doi: 10.1016/j.dib.2022.108644. eCollection 2022 Dec.

The complete chloroplast genome sequencing data of Juniperus sabina L. (Cupressaceae Bartl.) from Kazakhstan

Shyryn Almerekova  1 , Moldir Yermagambetova  1   2 , Saule Abugalieva  1   2 , Yerlan Turuspekov  1   2
Affiliations

The complete chloroplast genome sequencing data of Juniperus sabina L. (Cupressaceae Bartl.) from Kazakhstan

Shyryn Almerekova et al. Data Brief. .

Abstract

The genus Juniperus L. (Cupressaceae Bartl.) is consisting of about 75 species that are divided into sections Caryocedrus Endlicher, Sabina (Miller) Spach, and Juniperus (syn: sect. Oxycedrus Spach). Juniperus sabina L. from section Sabina is an important shrub for the maintenance of the ecosystem in mountainous regions and a source of medicinal compounds. The species is monoecious, rarely dioecious, and distributed in Europe, Central Asia, China, and Mongolia. The goal of the present study was to sequence and reconstruct the complete chloroplast genome of J. sabina. De novo chloroplast (cp) genome assembly for J. sabina was conducted using Illumina paired-end reads. The assembled cp genome size is 127,646 bp in length and has a typical circular DNA molecule. The genome encodes 118 genes, including 82 protein-coding genes, 32 tRNA genes, and 4 rRNA genes, the overall GC content is 34,36%. The complete cp genome nucleotide sequence of J. sabina was deposited to the NCBI (National Center for Biotechnology Information) under accession number OL467323. The raw data in fastq format was deposited to the NCBI sequence read archive under accession number SRR21515769.

Keywords: Chloroplast genome; Illumina sequencing; Juniperus sabina; Kazakhstan; Next-generation sequencing; Novaseq.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig 1
Fig. 1
Circular map of the J. sabina chloroplast genome.
Fig 2
Fig. 2
Neighbor joining of the concatenated phylogenetic tree based on matK and rbcL nucleotide sequences. denotes species analyzed in this study.
See this image and copyright information in PMC

References

    1. Doyle J.J., Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin. 1987;19:11–15.
    1. Bolger A.M., Lohse M., Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30:2114–2120. doi: 10.1093/bioinformatics/btu170. - DOI - PMC - PubMed
    1. Bankevich A., Nurk S., Antipov D., Gurevich A.A., Dvorkin M., Kulikov A.S., Lesin V.M., Nikolenko S.I., Pham S., Prjibelski A.D., Pyshkin A.V., Sirotkin A.V., Vyahhi N., Tesler G., Alekseyev M.A., Pevzner P.A. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 2012;19:455–477. doi: 10.1089/cmb.2012.0021. - DOI - PMC - PubMed
    1. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30:2068–2069. doi: 10.1093/bioinformatics/btu153. - DOI - PubMed
    1. Lohse M., Drechsel O., Kahlau S., Bock R. OrganellarGenomeDRAW - a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res. 2013;41:W575–W581. doi: 10.1093/nar/gkt289. - DOI - PMC - PubMed

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