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Review
. 2022 Nov 8:13:1043478.
doi: 10.3389/fpls.2022.1043478. eCollection 2022.

Plant-based expression platforms to produce high-value metabolites and proteins

Aditya Kulshreshtha  1 , Shweta Sharma  2 , Carmen S Padilla  1 , Kranthi K Mandadi  1   3   4
Affiliations
Review

Plant-based expression platforms to produce high-value metabolites and proteins

Aditya Kulshreshtha et al. Front Plant Sci. .

Abstract

Plant-based heterologous expression systems can be leveraged to produce high-value therapeutics, industrially important proteins, metabolites, and bioproducts. The production can be scaled up, free from pathogen contamination, and offer post-translational modifications to synthesize complex proteins. With advancements in molecular techniques, transgenics, CRISPR/Cas9 system, plant cell, tissue, and organ culture, significant progress has been made to increase the expression of recombinant proteins and important metabolites in plants. Methods are also available to stabilize RNA transcripts, optimize protein translation, engineer proteins for their stability, and target proteins to subcellular locations best suited for their accumulation. This mini-review focuses on recent advancements to enhance the production of high-value metabolites and proteins necessary for therapeutic applications using plants as bio-factories.

Keywords: heterologous production; molecular farming; plant secondary metabolites; plant-made secondary metabolites; plant-made therapeutic proteins.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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