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. 2023:2599:59-68.
doi: 10.1007/978-1-0716-2847-8_6.

Profiling Accessible Chromatin and Nucleosomes in the Mammalian Genome

Hee-Woong Lim  1 , Makiko Iwafuchi  2
Affiliations

Profiling Accessible Chromatin and Nucleosomes in the Mammalian Genome

Hee-Woong Lim et al. Methods Mol Biol. 2023.

Abstract

Genomic DNA wraps around core histones to form nucleosomes, which provides steric constraints on how transcription factors (TFs) can interact with gene regulatory sequences. It is increasingly apparent that well-positioned, accessible nucleosomes are an inherent feature of active enhancers and can facilitate cooperative TF binding, referred to as nucleosome-mediated cooperativity. Thus, profiling chromatin and nucleosome properties (accessibility, positioning, and occupancy) on the genome is crucial to understand cell-type-specific gene regulation. Here we describe a simplified protocol to profile accessible nucleosomes in the mammalian genome using low-level and high-level micrococcal nuclease (MNase) digestion followed by genome-wide sequencing.

Keywords: Accessible nucleosome; Chromatin accessibility; Fragile nucleosome; MNase-seq; Nucleosome occupancy; Nucleosome positioning.

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References

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