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Review
. 2022 Nov 19;27(22):8040.
doi: 10.3390/molecules27228040.

Collection of Hairy Roots as a Basis for Fundamental and Applied Research

Anna Yurievna Stepanova  1 , Maria Viktorovna Malunova  1 , Evgeny Aleksandrovich Gladkov  1 , Sergey Viktorovich Evsyukov  1 , Dmitry Viktorovich Tereshonok  1 , Aleksandra Ivanovna Solov'eva  1
Affiliations
Review

Collection of Hairy Roots as a Basis for Fundamental and Applied Research

Anna Yurievna Stepanova et al. Molecules. .

Abstract

Due to population growth, instability of climatic conditions, and reduction of the areas of natural ecosystems, it becomes necessary to involve modern biotechnological approaches to obtain highly productive plant material. This statement applies both to the creation of plant varieties and the production of new pharmaceutical raw materials. Genetic transformation of valuable medicinal plants using Agrobacterium rhizogenes ensures the production of stable and rapidly growing hairy roots cultures that have a number of advantages compared with cell culture and, above all, can synthesize root-specific substances at the level of the roots of the intact plant. In this regard, special attention should be paid to the collection of hairy roots of the Institute of Plant Physiology RAS, Russian Academy of Sciences, the founder of which was Dr. Kuzovkina I.N. Currently, the collection contains 38 hairy roots lines of valuable medicinal and forage plants. The review discusses the prospects of creating a hairy roots collection as a basis for fundamental research and commercial purposes.

Keywords: collection; hairy roots; secondary metabolites.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The main β-carboline alkaloids in roots of Peganum harmala.
Figure 2
Figure 2
Calli (left) and hairy roots (right) of Peganum harmala obtained in 1987. The photos were taken in 2021.
Figure 3
Figure 3
The hairy roots of Ruta graveolens (left). The main component of the essential oil of common rue is geijarene (right).
Figure 4
Figure 4
The examples of Ruta graveolens calli obtained during the period 1969–1976. The photos were taken in 2021.
Figure 5
Figure 5
The hairy roots of Rauwolfia serpentina (left) and the main alkaloid (right).
Figure 6
Figure 6
Hairy roots of Glycyrrhiza uralensis (left) and Rubia tinctorum (right). The photo was taken in 2021.
Figure 7
Figure 7
Hairy roots of Scutellaria baicalensis obtained in 1996 (left). The main root-specific flavones (right). The photo was taken in 2021.
Figure 8
Figure 8
The percentage of plant families represented in the collection of hairy roots.
Figure 9
Figure 9
Growth index of the Scutellaria baicalensis Georgi in in vitro cultures.
See this image and copyright information in PMC

References

    1. Chandran H., Meena M., Barupal T., Sharma K. Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnol. Rep. 2020;26:e00450. doi: 10.1016/j.btre.2020.e00450. - DOI - PMC - PubMed
    1. Nasri H., Baradaran A., Shirzad H., Kopaei M.R. New concepts in nutraceuticals as alternative for pharmaceuticals. Int. J. Prev. Med. 2014;5:1487–1499. - PMC - PubMed
    1. Quaglio D., Talib W.H., Daoud S., Mahmod A.I., Hamed R.A., Awajan D., Feras Abuarab S., Odeh L.H., Khater S., Al Kury L.T. Plants as a source of anticancer agents: From bench to bedside. Molecules. 2022;27:4818. doi: 10.3390/MOLECULES27154818. - DOI - PMC - PubMed
    1. Ramachandra Rao S., Ravishankar G.A. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv. 2002;20:101–153. doi: 10.1016/S0734-9750(02)00007-1. - DOI - PubMed
    1. Barrales-Cureño H.J., Valdivia A.C.R., Hernández M.S. Increased production of taxoids in suspension cultures of Taxus globosa after elicitation. Futur. Pharmacol. 2022;2:45–54. doi: 10.3390/futurepharmacol2010004. - DOI

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