Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Nov 21;27(22):8093.
doi: 10.3390/molecules27228093.

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

Marouane Mohaddab  1 , Younes El Goumi  2 , Monica Gallo  3 , Domenico Montesano  4 , Gokhan Zengin  5 , Abdelhakim Bouyahya  6 , Malika Fakiri  1
Affiliations
Review

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

Marouane Mohaddab et al. Molecules. .

Abstract

Medicinal plants are rich sources of bioactive compounds widely used as medicaments, food additives, perfumes, and agrochemicals. These secondary compounds are produced under stress conditions to carry out physiological tasks in plants. Secondary metabolites have a complex chemical structure with pharmacological properties. The widespread use of these metabolites in a lot of industrial sectors has raised the need to increase the production of secondary metabolites. Biotechnological methods of cell culture allow the conservation of plants, as well as the improvement of metabolite biosynthesis and the possibility to modify the synthesis pathways. The objective of this review is to outline the applications of different in vitro culture systems with previously reported relevant examples for the optimal production of plant-derived secondary metabolites.

Keywords: biological effects; cell culture; elicitor; secondary metabolites.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Principal biosynthetic pathways of major secondary metabolite plants’ classes.
See this image and copyright information in PMC

References

    1. Harborne J.B. Classes and Functions of Secondary Products from Plants. Chem. Plants. 1999;26:1–25.
    1. Aboukhalid K., Lamiri A., Agacka-Moldoch M., Doroszewska T., Douaik A., Bakha M., Casanova J., Tomi F., Machon N., Faiz C.A. Chemical Polymorphism of Origanum Compactum Grown in All Natural Habitats in Morocco. Chem. Biodivers. 2016;13:1126–1139. doi: 10.1002/cbdv.201500511. - DOI - PubMed
    1. Yang W.-C., Bao H.-Y., Liu Y.-Y., Nie Y.-Y., Yang J.-M., Hong P.-Z., Zhang Y. Depsidone Derivatives and a Cyclopeptide Produced by Marine Fungus Aspergillus unguis under Chemical Induction and by Its Plasma Induced Mutant. Molecules. 2018;23:2245. doi: 10.3390/molecules23092245. - DOI - PMC - PubMed
    1. Nasri H., Baradaran A., Shirzad H., Rafieian-Kopaei M. New Concepts in Nutraceuticals as Alternative for Pharmaceuticals. Int. J. Prev. Med. 2014;5:1487. - PMC - PubMed
    1. Elazzouzi H., Soro A., Elhilali F., Bentayeb A., El Belghiti M.A., Zair T. Phytochemical Study of Anacyclus pyrethrum (L.) of Middle Atlas (Morocco), and in Vitro Study of Antibacterial Activity of Pyrethrum. Adv. Nat. Appl. Sci. 2014;8:131–141.