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. 2022 Nov 14;14(22):4823.
doi: 10.3390/nu14224823.

Effects on Serum Inflammatory Cytokines of Cholecalciferol Supplementation in Healthy Subjects with Vitamin D Deficiency

Angelo Fassio  1 , Davide Gatti  1 , Maurizio Rossini  1 , Davide Bertelle  1 , Riccardo Bixio  1 , Ombretta Viapiana  1 , Stefano Milleri  2 , Camilla Benini  1 , Francesca Pistillo  1 , Giulia Zanetti  1 , Giovanni Adami  1
Affiliations

Effects on Serum Inflammatory Cytokines of Cholecalciferol Supplementation in Healthy Subjects with Vitamin D Deficiency

Angelo Fassio et al. Nutrients. .

Abstract

The effects of different cholecalciferol supplementation regimens on serum inflammatory cytokines in healthy subjects with vitamin D deficiency are still lacking. This is a single-center, open-label, randomized, parallel group study involving healthy subjects deficient in vitamin D (baseline 25OHD < 20 ng/mL) receiving oral cholecalciferol with three different dosing regimens: Group A: 10,000 IU/day for 8 weeks followed by 1000 IU/day for 4 weeks; Group B: 50,000 IU/week for 12 weeks and Group C: 100,000 IU every other week for 12 weeks. IL-17A, IL-6, IL-8, IL-10, IL-23 and TNFα were measured at baseline and at week 4, 8, 12, and 16. 75 healthy subjects were enrolled (58.7% female), with an average age of 34.1 ± 10.2 years. No statistical differences were observed among groups at baseline for either IL-6, IL-17A, IL-23, IL-8 or IL-10 at any time point; TNFα was indetectable. Concerning the whole sample, the time trend analysis showed a statistically significant linear trend for decreasing values over the treatment period for IL-6 (p = 0.016) and IL-17A (p = 0.006), while no significant time trends were observed for the other teste cytokines. No significant differences were found in the serum concentrations of the tested cytokines between week 12 and week 16. In young healthy individuals deficient in vitamin D, cholecalciferol administration showed a decrease in the serum IL-6 and IL-17A concentrations, without marked differences using the three regimens.

Keywords: autoimmune diseases; cholecalciferol; cytokines; immune response; osteoporosis; supplementation; vitamin D.

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Conflict of interest statement

Davide Gatti has received advisory board honoraria, consultancy fees and/or speaker fees from: Amgen, Celgene Eli-Lilly, MSD-Italia, Organon, UCB. Maurizio Rossini has received advisory board honoraria, consultancy fees and/or speaker fees from: Amgen, Abbvie, BMS, Eli-Lilly, Galapagos, Novartis, Pfizer, Sandoz, Theramex, UCB. All the other Authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Absolute changes with time trend analysis of the overall cohort over time of IL-6, IL-17A, IL-10, IL-8 and IL-23. Treatment was administered from baseline to week 12 (solid line), the follow-up period without supplementation (from week 12 to week 16) is depicted by the dashed line. * p < 0.05 with respect to baseline (one-way ANOVA for repeated measures). Error bars show 95% Confidence Interval.
See this image and copyright information in PMC

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