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. 2022 Nov 19;14(22):4902.
doi: 10.3390/nu14224902.

Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health

Alessandra Pino  1   2   3 , Amanda Vaccalluzzo  1 , Cinzia Caggia  1   2   3 , Silvia Balzaretti  4 , Luca Vanella  3   5 , Valeria Sorrenti  3   5 , Aki Ronkainen  6 , Reetta Satokari  6 , Cinzia Lucia Randazzo  1   2   3
Affiliations

Lacticaseibacillus rhamnosus CA15 (DSM 33960) as a Candidate Probiotic Strain for Human Health

Alessandra Pino et al. Nutrients. .

Abstract

Lactobacilli with probiotic properties have emerged as promising tools for both the prevention and treatment of vaginal dysbiosis. The present study aimed to study the in vitro probiotic potential of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain isolated from a healthy vaginal ecosystem. The strain was evaluated for both functional (antagonistic activity against pathogens; H2O2, organic acid, and lactic acid production; antioxidant and anti-inflammatory activities; ability to adhere to intestinal mucus and to both CaCo-2 and VK7/E6E7 cell lines; exopolysaccharide production; surface properties; and ability to survive during gastrointestinal transit) and safety (hemolytic, DNase, and gelatinase activities; mucin degradation ability; production of biogenic amines; and resistance to antimicrobials) characteristics. Data revealed that the tested strain was able to antagonize a broad spectrum of vaginal pathogens. In addition, the adhesion capacity to both vaginal and intestinal cell lines, as well as anti-inflammatory and antioxidant activities, was detected. The ability of the Lacticaseibacillus rhamnosus CA15 (DSM 33960) strain to survive under harsh environmental conditions occurring during the gastrointestinal passage suggests its possible oral delivery. Thus, in vitro data highlighted interesting probiotic properties of the CA15 (DSM 33960) strain, which could represent a valuable candidate for in vivo vaginal infections treatment.

Keywords: anti-inflammatory activity; antioxidant activity; functional features; lactobacilli; pathogen inhibition; safety.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. A.P., C.C. and C.L.R. declare that they are members of ProBioEtna, a spinoff of the University of Catania, Italy. S.B. is employed by Sacco Systems, Cadorago, Italy. The authors declare that they do not have any personal, financial, professional, political, or legal interests with a significant chance of interfering with the performance of their ethical or legal duties.

Figures

Figure 1
Figure 1
PFGE profiles of AscI-digested genomic DNA of L. rhamnsous CA15 (DSM 33960) strain. M: Lambda PFG marker (New England Biolabs, Inc., Ipswich, MA, USA).
Figure 2
Figure 2
Lipid peroxidation inhibitory activity of the L. rhamnsous CA15 (DSM 33960) strain.
Figure 3
Figure 3
DPPH scavenger and SOD-like activity explicated by the CA15 (DSM 33960) strain tested at different concentrations (3, 2.25, 1.5, and 0.75 mg/mL) (Panels A,B); LPS treatment (100 µg/mL) effect on mitochondrial membrane potential (Panel C) (** p < 0.005 vs. CTRL); Assessment of Caco-2 cell viability after treatments (Panel D); Measurement of IL-6 and IL-2 levels in cell supernatants by ELISA (Panels E,F) (* p < 0.05, *** p < 0.0005 vs. CTRL; and ### p < 0.0005 vs. LPS).
See this image and copyright information in PMC

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