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. 2022 Nov 12;14(22):4878.
doi: 10.3390/polym14224878.

Bioinspired Electropun Fibrous Materials Based on Poly-3-Hydroxybutyrate and Hemin: Preparation, Physicochemical Properties, and Weathering

Polina M Tyubaeva  1 , Ivetta A Varyan  1   2 , Anna K Zykova  1 , Alena Yu Yarysheva  3 , Pavel V Ivchenko  3 , Anatoly A Olkhov  1   2 , Olga V Arzhakova  3
Affiliations

Bioinspired Electropun Fibrous Materials Based on Poly-3-Hydroxybutyrate and Hemin: Preparation, Physicochemical Properties, and Weathering

Polina M Tyubaeva et al. Polymers (Basel). .

Abstract

The development of innovative fibrous materials with valuable multifunctional properties based on biodegradable polymers and modifying additives presents a challenging direction for modern materials science and environmental safety. In this work, high-performance composite fibrous materials based on semicrystalline biodegradable poly-3-hydroxybutyrate (PHB) and natural iron-containing porphyrin, hemin (Hmi) were prepared by electrospinning. The addition of Hmi to the feed PHB mixture (at concentrations above 3 wt.%) is shown to facilitate the electrospinning process and improve the quality of the electrospun PHB/Hmi materials: the fibers become uniform, their average diameter decreases down to 1.77 µm, and porosity increases to 94%. Structural morphology, phase composition, and physicochemical properties of the Hmi/PHB fibrous materials were studied by diverse physicochemical methods, including electronic paramagnetic resonance, optical microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, elemental analysis, differential scanning calorimetry, Fourier-transformed infrared spectroscopy, mechanical analysis, etc. The proposed nonwoven Hmi/PHB composites with high porosity, good mechanical properties, and retarded biodegradation due to high antibacterial potential can be used as high-performance and robust materials for biomedical applications, including breathable materials for wound disinfection and accelerated healing, scaffolds for regenerative medicine and tissue engineering.

Keywords: biodegradable polymers; electrospinning; hemin; new technologies; outdoor weathering; poly(3-hydroxybutyrate).

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structural formulae of PHB (A) and hemin (B).
Figure 2
Figure 2
The micrographs of Hmi/PHB fibrous nanocomposites with different content of hemin: 1 wt.% (A), 3 wt.% (B), and 5 wt.% (C).
Figure 3
Figure 3
SEM micrographs of PHB and Hmi/PHB nonwoven materials with different content of Hmi: (A) neat PHB, (B) Hmi/PHB with 1 wt.% of Hmi, (C) Hmi/PHB with 3 wt.%, (D) Hmi/PHB with 5 wt.% of Hmi.
Figure 4
Figure 4
EDX elemental analysis of iron atoms in the Hmi/PHB samples with different content of hemin: (A) 0 wt.%, (B) 1 wt.%, (C) 3 wt.%, (D) 5 wt.%.
Figure 5
Figure 5
Degree of crystallinity versus the content of Hmi in the Hmi/PHB system.
Figure 6
Figure 6
The DSC scans of Hmi/PHB with different content of Hmi: first heating run (A), second heating run (B).
Figure 7
Figure 7
Correlation times (A) and concentration of TEMPO (B) versus the content of Hmi in the Hmi/PHB material.
Figure 8
Figure 8
Snapshots and SEM images of the Hmi/PHB samples before (A) and after (B) outdoor exposure.
Figure 9
Figure 9
The FTIR spectra of pristine (blue line) and weathered (red line) Hmi/PHB samples.
Figure 10
Figure 10
DSC scans of hemin/PHB (5 wt.%) nanocomposites before (yellow line) and after weathering (blue line): (A) first heating run and (B) second heating run.
Figure 11
Figure 11
Strain-stress curves of the initial (blue line) and weathered (yellow line) samples.
See this image and copyright information in PMC

References

    1. Poltronieri P., Kumar P. Handbook of Ecomaterials. Springer; Cham, Switzerland: 2017. Polyhydroxyalcanoates (PHAs) in Industrial Applications; pp. 1–30. - DOI
    1. Rajan K.P., Thomas S.P., Gopanna A., Chavali M. Handbook of Ecomaterials. Springer International Publishing AG; Cham, Switzerland: 2017. Polyhydroxybutyrate (PHB): A Standout Biopolymer for Environmental Sustainability; pp. 1–23. - DOI
    1. Kostopoulos V., Kotrotsos A., Fouriki K., Kalarakis A., Portan D. Fabrication and Characterization of Polyetherimide Electrospun Scaffolds Modified with Graphene Nano-Platelets and Hydroxyapatite Nano-Particles. Int. J. Mol. Sci. 2020;21:583. doi: 10.3390/ijms21020583. - DOI - PMC - PubMed
    1. Dror Y., Salalha W., Khalfin R.L., Cohen Y., Yarin A.L., Zussman E. Carbon nanotubes embeded in oriented polymer nanofibers by electrospinning. Langmuir. 2003;19:7012–7020. doi: 10.1021/la034234i. - DOI - PubMed
    1. Moydeen M.A., Syed A.M., Padusha E.F., Aboelfetoh S.S., Al-Deyab M.H. Fabrication of electrospun poly(vinyl alcohol)/dextran nanofibers via emulsion process as drug delivery system: Kinetics and in vitro release study. Int. J. of Biol. Macromol. 2018;116:1250–1259. doi: 10.1016/j.ijbiomac.2018.05.130. - DOI - PubMed

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