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. 2023 Jun;68(3):395-402.
doi: 10.1007/s12223-022-01019-7. Epub 2022 Nov 25.

Deletion of the cheZ gene results in the loss of swimming ability and the decrease of adhesion ability to Caco-2 cells in Escherichia coli Nissle 1917

Bingming Ou  1   2 , Haihui Lv  1 , Hua Ge  1 , Dexiang Fu  1 , Xin Lin  1 , Saiqiao Huang  1 , Xiaojie Chen  1 , Yufu Liu  1 , Sainan Li  1   2 , Wenhua Liu  1 , Lihua Huang  1 , Ying Yang  3 , Minyu Zhang  4   5
Affiliations

Deletion of the cheZ gene results in the loss of swimming ability and the decrease of adhesion ability to Caco-2 cells in Escherichia coli Nissle 1917

Bingming Ou et al. Folia Microbiol (Praha). 2023 Jun.

Abstract

The aim of this study was to elucidate the biological functions of the motility regulatory protein CheZ in the probiotic strain Escherichia coli Nissle 1917. A cheZ gene deletion strain Nissle 1917ΔcheZ was constructed using the CRISPR/Cas9 two-plasmid system, and the corresponding complemented strain Nissle 1917ΔcheZ/pBR322-cheZ was established. Combined studies of growth kinetics testing, motility assays, swarming motility assays, and bacterial adherence assays were performed to study the motility regulatory protein CheZ-mediated functions in the prototype Nissle 1917 strain, its isogenic cheZ mutant, and the corresponding complemented strain. The growth rate of the cheZ mutant strain was lower than that of the wild-type strain in the exponential growth phase. The motility of the cheZ mutant strain was significantly lower than that of the wild-type strain. And the adhesion ability of ΔcheZ mutant to the Caco-2 cells was significantly lower than that of the wild-type strain and complemented strain. In conclusion, the results presented in our study suggested that the deletion of the cheZ gene in E. coli Nissle 1917 led to a significant reduction of its swimming ability and a subsequent marked decrease of adhesion to the Caco-2 cells.

Keywords: Adherence; CheZ; Growth kinetics; Nissle 1917; Swarming motility.

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