Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Nov 25;189(12):475.
doi: 10.1007/s00604-022-05560-7.

A sensitive multimode dot-filtration strip for the detection of Salmonella typhimurium using MoS2@Fe3O4

Luxiang Gao  1 , Xiaoyu Xu  1 , Wenxiu Liu  1 , Jinjuan Xie  1 , Hongyan Zhang  2 , Shuyuan Du  3
Affiliations

A sensitive multimode dot-filtration strip for the detection of Salmonella typhimurium using MoS2@Fe3O4

Luxiang Gao et al. Mikrochim Acta. .

Abstract

A sensitive, accurate, and rapid multimode dot-filtration immunoassay (MDFIA) was established for the detection of Salmonella typhimurium using the intrinsic color, catalytic property, and photothermal effect of magnetic molybdenum disulphide (MoS2@Fe3O4). The critical performance parameters of MDFIA were optimized in detail. The sensitivity of MDFIA can be improved by the catalytic color development and photothermal conversion of MoS2@Fe3O4 with a limit of detection (LOD) of 101 CFU·mL-1, which is an order of magnitude lower than direct visual detection (102 CFU·mL-1). Besides, the magnetic property of MoS2@Fe3O4 was used for the rapid enrichment and separation of the target allowing detection of trace concentrations of Salmonella typhimurium. The selectivity and applicability of the MDFIA were verified in spiked samples, indicating that the established assay may have bright application prospects for the detection and control of foodborne pathogens. A multimode dot-filtration immunoassay was constructed for Salmonella typhimurium rapid detection based on the peroxidase-like activity, magnetic property, and photothermal effect of MoS2@Fe3O4.

Keywords: Catalysis; Colorimetry; MoS2@Fe3O4; Multimode dot-filtration immunoassay; Photothermal effect; Salmonella typhimurium.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Tack DM, Ray L, Griffin PM, Cieslak PR, Dunn J, Rissman T, Jervis R, Lathrop S, Muse A, Duwell M, Smith K, Tobin-D’Angelo M, Vugia DJ, Zablotsky KJ, Wolpert BJ, Tauxe R, Payne DC (2020) Preliminary incidence and trends of infections with pathogens transmitted commonly through food - foodborne diseases active surveillance network, 10 U.S. Sites, 2016–2019. MMWR Morb Mortal Wkly Rep 69:509–514. https://doi.org/10.15585/mmwr.mm6917a1 - DOI
    1. Khan S, McWhorter AR, Moyle TS, Chousalkar KK (2021) Refrigeration of eggs influences the virulence of Salmonella typhimurium. Sci Rep 11:18026. https://doi.org/10.1038/s41598-021-97135-4 - DOI
    1. Pancza B, Szathmáry M, Gyurján I, Bánkuti B, Tudós Z, Szathmary S, Stipkovits L, Sipos-Kozma Z, Ásványi B, Varga L, Szenthe K, Bánáti F (2021) A rapid and efficient DNA isolation method for qPCR-based detection of pathogenic and spoilage bacteria in milk. Food Control 130:108236. https://doi.org/10.1016/j.foodcont.2021.108236 - DOI
    1. Hou Y, Tang W, Qi W, Guo X, Lin J (2020) An ultrasensitive biosensor for fast detection of Salmonella using 3D magnetic grid separation and urease catalysis. Biosens Bioelectron 157:112160. https://doi.org/10.1016/j.bios.2020.112160 - DOI
    1. Ma M, Zhao J, Yan X, Zeng Z, Wan D, Yu P, Xia J, Zhang G, Gong D (2022) Synergistic effects of monocaprin and carvacrol against Escherichia coli O157:H7 and Salmonella typhimurium in chicken meat preservation. Food Control 132:108480. https://doi.org/10.1016/j.foodcont.2021.108480 - DOI

Publication types