Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Feb 25;15(4):1387-99.
doi: 10.1093/nar/15.4.1387.

Assembly of pre-mRNA splicing complex is cap dependent

E Patzelt, E Thalmann, K Hartmuth, D Blaas, E Kuechler
Free PMC article

Assembly of pre-mRNA splicing complex is cap dependent

E Patzelt et al. Nucleic Acids Res. .
Free PMC article

Abstract

To study the influence of the ubiquitous cap structure of nuclear pre-mRNAs on the assembly of a functional splicing complex, the in vitro splicing of a truncated human metallothionein pre-mRNA was examined in the presence of the cap analogue m7GTP. Significant inhibition of splicing was observed at a concentration as low as 5 microM m7GTP. Analysis of the splicing reaction on glycerol density gradients showed two complexes sedimenting at 45S and 22S. When the reaction was carried out in presence of m7GTP a marked decrease of the material sedimenting at 45S, representing the active splicing complex, was observed. When capped pre-mRNA was replaced by uncapped pre-mRNA, complex formation was significantly reduced. These data indicate that the cap structure plays an important yet unknown role in the assembly of spliceosomes.

PubMed Disclaimer

References

    1. Cell. 1980 Jan;19(1):69-78 - PubMed
    1. Cell. 1986 Sep 12;46(6):845-55 - PubMed
    1. Nature. 1982 Oct 28;299(5886):797-802 - PubMed
    1. Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
    1. Nucleic Acids Res. 1983 Sep 10;11(17):5821-35 - PubMed

Publication types