Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2601:363-378.
doi: 10.1007/978-1-0716-2855-3_20.

Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research

Sina Schäkermann  1 , Pascal Dietze  2 , Julia E Bandow  2
Affiliations

Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research

Sina Schäkermann et al. Methods Mol Biol. 2023.

Abstract

Current research is focusing on ribosome heterogeneity as a response to changing environmental conditions and stresses. Altered stoichiometry and composition of ribosomal proteins as well as association of additional protein factors are mechanisms for shaping the protein expression profile or hibernating ribosomes. In this updated chapter, we present a method for the isolation of ribosomes to analyze antibiotic-induced changes in the composition of ribosomes in Bacillus subtilis or other bacteria. Ribosomes and associated proteins are isolated by ultracentrifugation, and proteins are identified and quantified using label-free mass spectrometry.

Keywords: Mass spectrometry; Proteomics; Ribosome heterogeneity; Stress.

PubMed Disclaimer

References

    1. McCoy LS, Xie Y, Tor Y (2011) Antibiotics that target protein synthesis. Wiley Interdiscip Rev RNA 2:209–232 - DOI - PubMed
    1. Wilson DN (2014) Ribosome-targeting antibiotics and mechanisms of bacterial resistance. Nat Rev Microbiol 12:35–48 - DOI - PubMed
    1. Silva JC, Gorenstein MV, Li G-Z et al (2006) Absolute quantification of proteins by LCMSE: a virtue of parallel MS acquisition. Mol Cell Proteomics 5:144–156 - DOI - PubMed
    1. Byrgazov K, Vesper O, Moll I (2013) Ribosome heterogeneity: another level of complexity in bacterial translation regulation. Curr Opin Microbiol 16:133–139 - DOI - PubMed - PMC
    1. Starosta AL, Lassak J, Jung K et al (2014) The bacterial translation stress response. FEMS Microbiol Rev 38:1172–1201 - DOI - PubMed

Publication types

LinkOut - more resources