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. 2022 Dec;67(6):723-726.
doi: 10.1165/rcmb.2022-0175LE.

Elexacaftor/Tezacaftor/Ivacaftor Disrupts Respiratory Tract Development in a Murine Fetal Lung Explant Model

Mickaël Lhuillier  1 , Laura Aoust  1   2 , Elise Dreano  1 , Marie-Laure Franco-Montoya  3 , Kim Landry-Truchon  4 , Nicolas Houde  4 , Stéphanie Chhun  5 , Alexandre Hinzpeter  1 , Aleksander Edelman  1 , Christophe Delacourt  1   6   7 , Lucie Jeannotte  4   8 , Isabelle Sermet-Gaudelus  1   2   6   7   9 , Alice Hadchouel  1   6   7
Affiliations

Elexacaftor/Tezacaftor/Ivacaftor Disrupts Respiratory Tract Development in a Murine Fetal Lung Explant Model

Mickaël Lhuillier et al. Am J Respir Cell Mol Biol. 2022 Dec.
No abstract available

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Figures

Figure 1.
Figure 1.
Effect of elexacaftor/tezacaftor/ivacaftor (ETI) on lung branching and bud dilation in lung explants from E12.5 Cftrtm1Eur/+ mouse embryos. (A) Lung explants from Cftrtm1Eur/+ embryos cultured for 3 days in Dulbecco's modified Eagle medium (DMEM)/F12 alone (A–D) (n = 31), or DMEM/F12 + ETI (E–H) (n = 9). Scale bar, 1 mm. (B) Number of terminal buds at T24, T48, and T72, expressed as the percentage increase in branching relative to baseline (T0). Data are expressed as the mean ± SEM and were analyzed by two-way ANOVA. ****P < 0.0001. (C) Number of terminal bud dilations at T72 relative to the mean number of terminal bud dilations in the medium-only control group. The number of terminal bud dilations is expressed as the mean ± SEM percentage of buds presenting an increase in diameter to over 2 SD from the mean value for the control group. Two group comparisons were performed in Mann-Whitney U tests. ****P < 0.0001.
See this image and copyright information in PMC

References

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