Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan;52(1):29-36.
doi: 10.1111/jop.13392. Epub 2022 Dec 13.

Saliva-based cell-free DNA and cell-free mitochondrial DNA in head and neck cancers have promising screening and early detection role

Lana Sayal  1 , Omar Hamadah  2 , Aroub Almasri  3 , Majdy Idrees  4 , Peter Thomson  5 , Omar Kujan  4
Affiliations

Saliva-based cell-free DNA and cell-free mitochondrial DNA in head and neck cancers have promising screening and early detection role

Lana Sayal et al. J Oral Pathol Med. 2023 Jan.

Abstract

Background: Cell-free DNA (cfDNA) and cell-free mitochondrial DNA (cf-mtDNA) have been postulated as potential diagnostic and prognostic biomarkers for different human malignancies. Early detection of head and neck malignancies is fundamental for optimal patient management. This study, therefore, aimed to assess the utility of saliva-based liquid biopsy as a noninvasive source of cfDNA and cf-mtDNA for detecting head and neck cancer (HNSCC).

Methods: One hundred thirty-three patients diagnosed with either oral leukoplakia (OLK) or HNSCC were compared with 137 healthy volunteers. An unstimulated whole saliva sample was collected from each participant. The absolute copy numbers of salivary cf-mtDNA and cfDNA were quantified using Multiplex Quantitative PCR. Two diagnostic indices based on the investigated molecules were assessed for their ability to differentiate between different diagnostic categories.

Results: The median scores of cfDNA and cf-mtDNA were statistically significantly higher among HNSCC patients (p < 0.05), revealing area under the curve values of 0.758 and 0.826, respectively. The associated accuracy for this test in discriminating HNSCC from other diagnostic categories was 77.37% for the cfDNA-based index and 80.5% for the cf-mtDNA-based index. The median score of cfDNA was statistically significantly higher for patients with severe epithelial dysplasia (OED) compared to those with epithelial keratosis with no OED and mild OED. However, there was no significant difference between controls and OLK individuals.

Conclusion: cfDNA and cf-mtDNA showed potential for use as precision medicine tools to detect HNSCC. Further multi-centre prospective studies are warranted to assess the prognostic utility of these molecules.

Keywords: cell-free DNA; cell-free mitochondrial DNA; head and neck cancer; liquid biopsy; precision medicine.

PubMed Disclaimer

Conflict of interest statement

Authors declare no conflict of interests.

Figures

FIGURE 1
FIGURE 1
The associations between the three diagnostic categories with A: the median score of cfDNA, B: the median score of cf‐mtDNA. **** p < 0.0005
FIGURE 2
FIGURE 2
The median score of cf‐mtDNA among the participants in the different diagnostic categories based on their smoking status. * p < 0.05, ** p < 0.005
FIGURE 3
FIGURE 3
The ROC curves for the utility of the proposed diagnostic indices in the discrimination between HNSCC and other diagnostic categories, (A) cfDNA‐based index, (B) cf‐mtDNA‐based index
See this image and copyright information in PMC

References

    1. Johnson DE, Burtness B, Leemans CR, Lui VWY, Bauman JE, Grandis JR. Head and neck squamous cell carcinoma. Nat Rev Dis Primers. 2020;6(1):92. - PMC - PubMed
    1. Surveillance, Epidemiology, and End Results (SEER) . Surveillance, Epidemiology, and End Results (SEER) Program‐ SEER*Stat Database. 2000. –2019. Accessed October 28, 2022. www.seer.cancer.gov.
    1. Patel A, Patel S, Patel P, Tanavde V. Saliva based liquid biopsies in head and neck cancer: how far are we from the clinic? Front Oncol. 2022;12:828434. - PMC - PubMed
    1. Gaballah K, Faden A, Fakih FJ, Alsaadi AY, Noshi NF, Kujan O. Diagnostic accuracy of Oral cancer and suspicious malignant mucosal changes among future dentists. Healthcare (Basel). 2021;9(3). - PMC - PubMed
    1. Grafton‐Clarke C, Chen KW, Wilcock J. Diagnosis and referral delays in primary care for oral squamous cell cancer: a systematic review. Br J Gen Pract. 2019;69(679):e112‐e126. - PMC - PubMed

MeSH terms

Grants and funding