Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb;50(2):1385-1391.
doi: 10.1007/s11033-022-08141-2. Epub 2022 Dec 5.

Correlated downregulation of VDR and CYP3A4 in colorectal cancer

Hossein Sadeghi  1 , Veys Hashemnia  2 , Ehsan Nazemalhosseini-Mojarad  3 , Mohammad Reza Ghasemi  2 , Reza Mirfakhraie  4   5
Affiliations

Correlated downregulation of VDR and CYP3A4 in colorectal cancer

Hossein Sadeghi et al. Mol Biol Rep. 2023 Feb.

Abstract

Background: The evidence obtained from experimental studies suggests the tumor-suppressive effects of vitamin D by controlling the differentiation, proliferation, and apoptosis in cancerous cells. Furthermore, the deregulation of genes involved in vitamin D metabolism has been reported in several types of cancer.

Methods: In the present study, we investigated the expression level of vitamin D metabolic pathway genes, including VDR, CYP3A4, RXRα, and GC, in colorectal cancer (CRC) samples compared with the adjacent tissues by using quantitative RT-PCR.

Results: The results indicated significant downregulation of CYP3A4 and VDR genes in CRC tissues compared with the adjacent control tissues (p < 0.01). RXRA and GC expression levels did not show any significant alteration among the studied samples. Moreover, a positive correlation was observed between the expression level of CYP3A4 and VDR genes (p < 0.0001). ROC curve analysis also revealed the potential diagnostic power of CYP3A4 and VDR genes in CRC samples.

Conclusion: Reduction in the expression of both CYP3A4 and VDR plays an important role in CRC due to the possible impairment in vitamin D metabolism. Further studies concerning the relationship between the expression of these genes and colorectal cancer pathogenesis and treatment are recommended.

Keywords: CYP3A4; Colorectal cancer; Expression; GC; RXRα; VDR; Vitamin D.

PubMed Disclaimer

References

    1. Sawicki T, Ruszkowska M, Danielewicz A, Niedźwiedzka E, Arłukowicz T, Przybyłowicz KE (2021) A review of colorectal cancer in terms of epidemiology, risk factors, development, symptoms and diagnosis. Cancers 13(9):2025 - DOI
    1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A et al (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: Cancer J Clin 71(3):209–249
    1. Rawla P, Sunkara T, Barsouk A (2019) Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors. Przeglad Gastroenterologiczny 14(2):89
    1. Dolatkhah R, Somi MH, Bonyadi MJ, Asvadi Kermani I, Farassati F, Dastgiri S (2015) Colorectal cancer in Iran: molecular epidemiology and screening strategies. J Cancer Epidemiol 2015:1–10 - DOI
    1. Hong SN (2018) Genetic and epigenetic alterations of colorectal cancer. Intest Res 16(3):327–337. https://doi.org/10.5217/ir.2018.16.3.327 - DOI

LinkOut - more resources