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. 1987 Apr;74(2):129-35.
doi: 10.1007/BF00224950.

Characterization of wheat germ initiation factor eIF-2

Characterization of wheat germ initiation factor eIF-2

R D Clarke et al. Mol Cell Biochem. 1987 Apr.

Abstract

The initiation factor eIF-2 that specifically binds Met-tRNAf and GTP in ternary complex (eIF-2. GTP. Met-tRNAf) has been purified to apparent homogeneity from wheat germ ribosomal salt wash. The purified factor exhibits a sedimentation coefficient of 5 X 5S and an aggregate molecular weight of 122,000-daltons for the native protein.

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