Characterization of wheat germ initiation factor eIF-2
- PMID: 3648477
- DOI: 10.1007/BF00224950
Characterization of wheat germ initiation factor eIF-2
Abstract
The initiation factor eIF-2 that specifically binds Met-tRNAf and GTP in ternary complex (eIF-2. GTP. Met-tRNAf) has been purified to apparent homogeneity from wheat germ ribosomal salt wash. The purified factor exhibits a sedimentation coefficient of 5 X 5S and an aggregate molecular weight of 122,000-daltons for the native protein.
Similar articles
-
Factors from wheat germ that enhance the activity of eukaryotic initiation factor eIF-2. Isolation and characterization of Co-eIF-2 beta.J Biol Chem. 1982 Jul 25;257(14):8233-7. J Biol Chem. 1982. PMID: 6919542
-
Protein synthesis in brine shrimp embryos. Regulation of the formation of the ternary complex (Met-tRNAf X eIF-2 X GTP) by two purified protein factors and phosphorylation of Artemia eIF-2.Eur J Biochem. 1981 Jul;117(3):543-51. Eur J Biochem. 1981. PMID: 6912815
-
Characterization of initiation factor eIF-3 from wheat germ.J Biol Chem. 1981 Feb 25;256(4):1582-6. J Biol Chem. 1981. PMID: 6906354
-
Molecular mechanisms of control of protein biosynthesis.Eur J Cell Biol. 1981 Dec;26(1):212-6. Eur J Cell Biol. 1981. PMID: 7035174 Review.
-
Phosphorylation reactions that influence the activity of elF-2.Curr Top Cell Regul. 1981;20:185-203. doi: 10.1016/b978-0-12-152820-1.50009-2. Curr Top Cell Regul. 1981. PMID: 6276082 Review. No abstract available.
Cited by
-
Identification of Aspartyl-tRNA Synthetase as Co-purifying with Wheat Germ eIF2.MicroPubl Biol. 2025 Jul 9;2025:10.17912/micropub.biology.001594. doi: 10.17912/micropub.biology.001594. eCollection 2025. MicroPubl Biol. 2025. PMID: 40756276 Free PMC article.
-
The plant translational apparatus.Plant Mol Biol. 1996 Oct;32(1-2):107-44. doi: 10.1007/BF00039380. Plant Mol Biol. 1996. PMID: 8980477 Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Miscellaneous