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. 2022 Dec 9;11(1):155.
doi: 10.1186/s13756-022-01194-9.

Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Nicoletta Formenti  1 , Flavia Guarneri  1 , Cristina Bertasio  1 , Giovanni Parisio  1 , Claudia Romeo  2 , Federico Scali  1 , Laura Birbes  1 , Maria Beatrice Boniotti  1 , Giuseppe Diegoli  3 , Loredana Candela  4 , Gianluca Antonio Romeo  4   5 , Paolo Pasquali  6 , Giovanni Loris Alborali  1
Affiliations

Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Nicoletta Formenti et al. Antimicrob Resist Infect Control. .

Abstract

Wastewater-based surveillance enabled the first detection of the mobile colistin resistance gene mcr-10 in Italy. This plasmid-borne resistance gene was found in strains of Klebsiella quasipneumoniae isolated from samples of human raw sewage collected over several months. Although the isolates were phenotypically susceptible to colistin, the emergence of mcr-10 is concerning due to the highly variable expression of the gene and the potential for horizontal transfer to other species. In addition, the strains also carried an extended-spectrum β-lactamase gene and were phenotypically resistant to several beta-lactams. This study highlights the value of wastewater-based surveillance as an effective tool to monitor the emergence of antimicrobial resistance in strains circulating in the community and the environment.

Keywords: AMR surveillance; Antimicrobial resistance; Colistin; Klebsiella quasipneumoniae similpneumoniae; Plasmid gene; Wastewater.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Genetic environment surrounding mcr-10.1—Alignment of the mcr-10.1-containing contigs of the three Klebsiella quasipneumoniae strains isolated from wastewater. Annotated regions are shown as oriented arrows according to the coding direction. Predicted functions are indicated with colour-coded keys
See this image and copyright information in PMC

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