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. 2023 Jan;9(1):82-90.
doi: 10.1002/vms3.1036. Epub 2022 Dec 10.

Molecular screening of SARS-CoV-2 in dogs and cats from households with infected owners diagnosed with COVID-19 during Delta and Omicron variant waves in Iran

Baharak Akhtardanesh  1 , Maziar Jajarmi  2 , Mohammadreza Shojaee  3 , Sina Salajegheh Tazerji  4   5 , Maziar Khalili Mahani  1 , Pouneh Hajipour  1 , Rasha Gharieb  6
Affiliations

Molecular screening of SARS-CoV-2 in dogs and cats from households with infected owners diagnosed with COVID-19 during Delta and Omicron variant waves in Iran

Baharak Akhtardanesh et al. Vet Med Sci. 2023 Jan.

Abstract

Objective: The emergence of SARS-CoV-2 infection in dogs and cats in different countries worldwide raises concerns that pets are at a higher risk for spreading or transmitting of SARS-CoV-2 to humans and other pets and increased the research works about the zoonotic aspects and natural routes of infection in companion animals. The current study aimed to detect the SARS-CoV-2 in household dogs and cats living with COVID-19 positive owners.

Methods: Deep oropharyngeal and rectal swabs were collected from 30 household pets (20 cats and 10 dogs) living with COVID-19 positive owners from April 2021 to 2022 in Kerman, Iran. All dogs' and cats' samples were tested by real-time reverse transcription polymerase chain reaction for detection of SARS-CoV-2.

Results: Two household cats out of 20 examined (10%) were positive for SARS-CoV-2, whereas none of the examined dogs were positive for SARS-CoV-2. The two cats positive for SARS-CoV-2 were symptomatic and suffered from severe anorexia with maximum contact with their infected owners.

Conclusion: This study reported the presence of SARS-CoV-2 in household cats in close contact with COVID-19 positive owners during the circulation of new SARS-CoV-2 variants (Delta and Omicron) in Iran and suggested that the transmission may have occurred from owners to their cats. Therefore, infected owners should eagerly limit close contact with their pets during COVID-19 illness.

Keywords: One Health; SARS-CoV-2 delta and Omicron variant; pet owners; pets.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIGURE 1
FIGURE 1
RT‐qPCR amplification curves for SARS‐CoV‐2 in cat positive samples. Green: sample 4, purple: sample 9, and yellow: positive control. (a) Amplification curves with Texas Red fluorophore for RdRp gene [Ct: 20.36 (sample 4), 21.56 (sample 9), and 30.14 (positive control)]. (b) Amplification curves with FAM fluorophore for N gene [Ct: 17.50 (sample 4), 19.89 (sample 9), and 29.93 (positive control)]. (c) Amplification curves with Hex fluorophore for RNaseP gene [Ct: 25.60 (sample 9), 27.90 (sample 4), and 29.99 (positive control)]
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Supplementary concepts