Removal of Zearalenone from Degummed Corn Oil by Hydrolase on a Batch-Refining Unit

Abstract

The removal of zearalenone (ZEN) from degummed corn oil (DCO) using hydrolase on a batch-refining unit was studied. According to single-factor and response surface experiments, the optimum technological conditions for reaching the maximum degradation rate were a temperature of 39.01 °C, a pH of 8.08, a time of 3.9 h, and an enzyme dosage of 44.7 mg/kg, whereby the rate of ZEN degradation can reach 94.66%. Different effects on the removal of ZEN were observed at different initial ZEN contents under the optimal technological conditions, of which the decrease was rapid for high ZEN content and slow for low ZEN content.

Keywords: degradation rate; degummed corn oil; hydrolase; response surface experiment; zearalenone.

Figure 1

Effects of reaction temperature on the degradation of zearalenone.

Figure 2

Effects of reaction pH on the degradation of zearalenone.

Figure 3

Effects of reaction time on the degradation of zearalenone.

Figure 4

Effects of enzyme dosage on the degradation of zearalenone.

Figure 5

Temperature, pH, and their interaction vs. the response surface and contour line of the degradation rate of ZEN.

Figure 6

Temperature, time, and their interaction vs. the response surface and contour line of the degradation rate of ZEN.

Figure 7

Temperature, enzyme dosage, and their interaction vs. the response surface and contour line of the degradation rate of ZEN.

Figure 8

PH, time, and their interaction vs. the response surface and contour line of the degradation rate of ZEN.

Figure 9

PH, enzyme dosage, and their interaction vs. the response surface and contour line of the degradation rate of ZEN.

Figure 10

Time, enzyme dosage, and their interaction vs. the response surface and contour line of the degradation rate of ZEN.

Figure 11

The effect of hydrolase dosage on the degradation of ZEN with different initial contents of ZEN in DCO.