Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec 1;11(23):3878.
doi: 10.3390/foods11233878.

Lipid and Protein Oxidation of Brown Rice and Selenium-Rich Brown Rice during Storage

Minghui Zhang  1 , Kunlun Liu  1   2
Affiliations

Lipid and Protein Oxidation of Brown Rice and Selenium-Rich Brown Rice during Storage

Minghui Zhang et al. Foods. .

Abstract

Selenium-rich rice has become one of the effective ways to increase people's selenium intake. Selenium-containing proteins have higher antioxidant properties, which may lead to selenium-rich brown rice (Se-BR) having better storage stability than ordinary brown rice (BR). By measuring the peroxidation value, fatty acid value, carbonyl value and protein secondary structure, it was found that Se-BR had higher oxidation resistance stability than BR. The biological function of the differential proteins (DEPs) between ordinary brown rice stored for 0 days (BR-0) and 180 days (BR-6) as well as Se-rich brown rice stored for 0 days (Se-0) and 180 days (Se-6) was investigated by using iTRAQ. A total of 237, 235, 113 and 213 DEPs were identified from group A (BR-0/BR-6), group B (Se-0/Se-6), group C (BR-0/Se-0) and group D (BR-6/Se-6), respectively. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEPs were mainly enriched in glucose metabolism, tricarboxylic acid cycle, fatty acid biosynthesis and degradation, glutathione metabolism, sulfur metabolism, peroxisome and other metabolic pathways. This study provides theoretical support for the study of protein oxidation kinetics and storage quality control of brown rice during storage.

Keywords: metabolic pathway; oxidation resistance; proteomics; storage protein; sulfur metabolism.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Peroxide value of ordinary brown rice (BR) and selenium-rich brown rice (Se-BR). Each data point is expressed as the mean and standard error of three replicates.
Figure 2
Figure 2
Fatty acid value of BR and Se-BR. Each data point is expressed as the mean and standard error of three replicates.
Figure 3
Figure 3
Carbonyl value of BR and Se-BR. Each data point is expressed as the mean and standard error of three replicates.
Figure 4
Figure 4
DEPs in the expression of Se-BR and BR in different metabolic pathways. Different metabolic pathways are distinguished by different colored boxes. Metabolic process substances are connected by arrows, and the direction of the arrow represents the direction of metabolism. Upregulated and downregulated proteins are marked in red and blue, respectively.
See this image and copyright information in PMC

References

    1. Liu K., Zhao Y., Chen F., Gu Z., Bu G. Purification, identification, and in vitro antioxidant activities of selenium-containing proteins from selenium-enriched brown rice. Eur. Food Res. Technol. 2011;234:61–68. doi: 10.1007/s00217-011-1609-3. - DOI
    1. Liu K., Ning M. Antioxidant activity stability and digestibility of protein from Se-enriched germinated brown rice. LWT. 2021;142:111032. doi: 10.1016/j.lwt.2021.111032. - DOI
    1. Xia X., Zhang X., Liu M., Duan M., Zhang S., Wei X., Liu X. Toward improved human health: Efficacy of dietary selenium on immunity at the cellular level. Food Funct. 2021;12:976–989. doi: 10.1039/D0FO03067H. - DOI - PubMed
    1. Zhang L., Zeng H., Cheng W.H. Beneficial and paradoxical roles of selenium at nutritional levels of intake in healthspan and longevity. Free Radic. Biol. Med. 2018;127:3–13. doi: 10.1016/j.freeradbiomed.2018.05.067. - DOI - PubMed
    1. Radomska D., Czarnomysy R., Radomski D., Bielawska A., Bielawski K. Selenium as a bioactive micronutrient in the human diet and its cancer chemopreventive activity. Nutrients. 2021;13:1649. doi: 10.3390/nu13051649. - DOI - PMC - PubMed

LinkOut - more resources