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. 2023 Dec;17(1):1-13.
doi: 10.1080/19336918.2022.2154732.

Dynamized ultra-low dilution of Ruta graveolens disrupts plasma membrane organization and decreases migration of melanoma cancer cell

Camille Fuselier  1 , Eleonore Dufay  2 , Alexandre Berquand  3 , Christine Terryn  4 , Arnaud Bonnomet  4 , Michael Molinari  5 , Laurent Martiny  2 , Christophe Schneider  2
Affiliations

Dynamized ultra-low dilution of Ruta graveolens disrupts plasma membrane organization and decreases migration of melanoma cancer cell

Camille Fuselier et al. Cell Adh Migr. 2023 Dec.

Abstract

Cutaneous melanoma is a cancer with a very poor prognosis mainly because of metastatic dissemination and therefore a deregulation of cell migration. Current therapies can benefit from complementary medicines as supportive care in oncology. In our study, we show that a dynamized ultra-low dilution of Ruta Graveolens leads to an in vitro inhibition of migration on fibronectin of B16F10 melanoma cells, as well as a decrease in metastatic dissemination in vivo. These effects appear to be due to a disruption of plasma membrane organization, with a change in cell and membrane stiffness, associated with a disorganization of the actin cytoskeleton and a modification of the lipid composition of the plasma membrane. Together, these results demonstrate, in in vitro and in vivo models of cutaneous melanoma, an anti-cancer and anti-metastatic activity of ultra-low dynamized dilution of Ruta graveolens and reinforce its interest as complementary medicine in oncology.

Keywords: Melanoma; cancer; dynamized ultra-low dilution; migration; plasma membrane.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Figures

Figure 1.
Figure 1.
Influence of Ruta Graveolens 9CH on cell stiffness of living individual B16F10 cells.
Figure 2.
Figure 2.
Effects of Ruta Graveolens 9CH on phospholipid membrane organization.
Figure 3.
Figure 3.
Effects of Ruta Graveolens 9CH on cholesterol.
Figure 4.
Figure 4.
Effects of Ruta Graveolens 9CH on induced-calcium flows.
Figure 5.
Figure 5.
Effects of Ruta Graveolens 9CH on actine organization.
Figure 6.
Figure 6.
Effects of Ruta Graveolens 9CH on B16F10 2D dispersed migration, 3D migration and circularity.
Figure 7.
Figure 7.
Effects of Ruta Graveolens 9CH on in vivo lung metastasis and survival.
See this image and copyright information in PMC

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