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. 2022 Nov 28:6:100399.
doi: 10.1016/j.crfs.2022.11.018. eCollection 2023.

Preparation and characterization of high embedding efficiency epigallocatechin-3-gallate glycosylated nanocomposites

Jianyong Zhang  1   2 , Hongchun Cui  3 , Jiahuan Qiu  2 , Yixin Zhong  2 , Caiping Yao  2 , Lanying Yao  2 , Qunxiong Zheng  2 , Chunhua Xiong  2
Affiliations

Preparation and characterization of high embedding efficiency epigallocatechin-3-gallate glycosylated nanocomposites

Jianyong Zhang et al. Curr Res Food Sci. .

Abstract

Glycosylated protein nano encapsulation was an efficient encapsulation technology, but its embedding rate for EGCG was not high, and the research on the embedding mechanism was relatively weak. Based on this, this study compared the embedding effect of glycosylated peanut globulin and glycosylated casein on EGCG. The embedding mechanism of EGCG with glycosylated protein was discussed by ultraviolet, fluorescence, infrared and fluorescence microscopy. Results revealed that the highest encapsulation efficiency of EGCG was 93.89 ± 1.11%. The neutral pH value and 0.3 mg/mL EGCG addition amount were suitable for EGCG glycosylated nanocomposites. The hydrogen bond between EGCG hydroxyl group and tyrosine and tryptophan of glycosylated protein is mainly non covalent. The encapsulation effect of EGCG glycosylated nanocomposites could be quenched by changing the polar environment and spatial structure of the group. The fluorescence characteristic and dispersibility of EGCG glycosylated peanut globin were higher than EGCG glycosylated casein. This study might provide a theoretical basis for EGCG microencapsulation technology and EGCG application in tea beverage and liquid tea food systems.

Keywords: Carboxymethyl chitosan; Casein; Epigallocatechin-3-gallate; Glycosylation; Peanut globin.

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Conflict of interest statement

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jianyong Zhang reports financial support was provided by 10.13039/100010754Zhejiang Gongshang University.

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
The average size and PDI of Ara-CMCS-EGCG (a) and Cas-CMCS-EGCG (b), zeta-potential of Ara-CMCS-EGCG (c) and Cas-CMCS-EGCG (d) with different pH. Note: PDI was polydisperse index, Ara-CMCS-EGCG was EGCG peanut globulin glycosylation copolymer, Cas-CMCS-EGCG was EGCG casein glycosylation copolymer.
Fig. 2
Fig. 2
The average size and PDI of Ara-CMCS-EGCG (a) and Cas-CMCS-EGCG (b), zeta-potential of Ara-CMCS-EGCG (c) and Cas-CMCS-EGCG (d) with different mass of EGCG addition. Note: PDI was polydisperse index, Ara-CMCS-EGCG was EGCG peanut globulin glycosylation copolymer, Cas-CMCS-EGCG was EGCG casein glycosylation copolymer.
Fig. 3
Fig. 3
The fluorescence emission spectra of Ara-CMCS-EGCG at 295 nm (a) and 280 nm (b), Cas-CMCS-EGCG at 295 nm (c) and 280 nm (d) with different mass of EGCG addition. Note: Ara-CMCS-EGCG was EGCG peanut globulin glycosylation copolymer, Cas-CMCS-EGCG was EGCG casein glycosylation copolymer.
Fig. 4
Fig. 4
FT-IR spectra of Ara-CMCS-EGCG and Cas-CMCS-EGCG complex. Note: FT-IR was Fourier transform infrared spectoscopy, Ara-CMCS-EGCG was EGCG peanut globulin glycosylation copolymer, Cas-CMCS-EGCG was EGCG casein glycosylation copolymer.
Fig. 5
Fig. 5
Nanostructures of Ara-CMCS-EGCG (a) and Cas-CMCS-EGCG (b) under the fluorescence microscopy. Note: Ara-CMCS-EGCG was EGCG peanut globulin glycosylation copolymer, Cas-CMCS-EGCG was EGCG casein glycosylation copolymer.
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