Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2592:21-36.
doi: 10.1007/978-1-0716-2807-2_2.

Uncommon Transplantation Sites: Transplantation of Islets and Islet Organoids in the Anterior Chamber of the Eye of Rodents and Monkeys

Tilo Moede  1 , Sai Bo Bo Tun  1   2 , Ingo B Leibiger  1 , Per-Olof Berggren  3   4   5
Affiliations

Uncommon Transplantation Sites: Transplantation of Islets and Islet Organoids in the Anterior Chamber of the Eye of Rodents and Monkeys

Tilo Moede et al. Methods Mol Biol. 2023.

Abstract

The anterior chamber of the eye is a highly vascularized and innervated location that is also particularly rich in oxygen and immune privileged. This uncommon transplantation site offers unique possibilities for the observation of the transplanted material as well as for local pharmacological intervention. Transplantation of islets and islet organoids to the anterior chamber of the eye of mice and monkeys facilitates a multitude of new approaches for research into islet physiology and pathophysiology and for the treatment of diabetes. We now present a short overview of the experimental possibilities and describe an updated protocol for transplantation of islets and islet organoids into mice and monkeys.

Keywords: Islet; Islet organoids; Monkey; Mouse; Nonhuman primate; Rodent; Transplantation.

PubMed Disclaimer

References

    1. van Dooremaal JC, van Dooremaal DJC (1873) Die Entwicklung der in fremden Grund versetzten lebenden Gewebe. Albr von Graefes Arch fur Klin und Exp Ophthalmol 19:359–373. https://doi.org/10.1007/BF01693910 - DOI
    1. Falck B (1959) Site of production of oestrogen in the ovary of the rat. Nature 184:1082. https://doi.org/10.1038/1841082a0 - DOI
    1. Kistler AD, Caicedo A, Abdulreda MH et al (2014) In vivo imaging of kidney glomeruli transplanted into the anterior chamber of the mouse eye. Sci Rep 4:3872. https://doi.org/10.1038/srep03872 - DOI
    1. Moore RA, Rosenblum HB, Tolins SH, Melchionna RH (1937) The physiological response of prostatic and vesicular transplants in the anterior chamber of the eye. J Exp Med 66:281–289. https://doi.org/10.1084/jem.66.3.281 - DOI
    1. Taylor D, Seiger A, Freedman R et al (1978) Electrophysiological analysis of reinnervation of transplants in the anterior chamber of the eye by the autonomic ground plexus of the iris. Proc Natl Acad Sci U S A 75:1009–1012. https://doi.org/10.1073/pnas.75.2.1009 - DOI

LinkOut - more resources