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. 2023 Jan;37(1):e24804.
doi: 10.1002/jcla.24804. Epub 2022 Dec 12.

Association of AXIN1 rs12921862 C/A and rs1805105 G/A and CTSB rs12898 G/A polymorphisms with papillary thyroid carcinoma: A case-control study

Shaghayegh Saljooghi  1 , Zahra Heidari  2 , Mohsen Saravani  1   3 , Mahnaz Rezaei  1   3 , Saeedeh Salimi  4
Affiliations

Association of AXIN1 rs12921862 C/A and rs1805105 G/A and CTSB rs12898 G/A polymorphisms with papillary thyroid carcinoma: A case-control study

Shaghayegh Saljooghi et al. J Clin Lab Anal. 2023 Jan.

Abstract

Background: Papillary thyroid cancer (PTC) is the most common type of thyroid cancer which its precise etiology remains unknown. However, environmental and genetic factors contribute to the etiology of PTC. Axis inhibition protein 1 (Axin1) is a scaffold protein that exerts its role as a tumor suppressor. In addition, Cathepsin B (Ctsb) is a cysteine protease with higher expression in several types of tumors. Therefore, the aim of this study was to investigate the possible association of AXIN1 rs12921862 C/A and rs1805105 G/A and CTSB rs12898 G/A polymorphisms with PTC susceptibility.

Materials & methods: In total, 156 PTC patients and 158 sex-, age-, and BMI-matched control subjects were enrolled in the study. AXIN1 rs12921862 C/A and rs1805105 G/A and CTSB rs12898 G/A polymorphisms were genotyped using the PCR-RFLP method.

Results: There was a relationship between AXIN1 rs12921862 C/A polymorphism and an increased risk of PTC in all genetic models except the overdominant model. The AXIN1 rs1805105 G/A polymorphism was associated with an increased PTC risk only in codominant and overdominant models. The frequency of AXIN1 Ars12921862 Ars1805105 haplotype was higher in the PTC group and also this haplotype was associated with an increased risk of PTC. Moreover, the AXIN1 rs12921862 C/A polymorphism was not associated with PTC clinical and pathological findings, but AXIN1 rs1805105 G/A polymorphism was associated with almost three folds of larger tumor size (≥1 cm). There was no association between CTSB rs12898 G/A polymorphism and PTC and its findings.

Conclusion: The AXIN1 rs12921862 C/A and rs1805105 G/A polymorphisms were associated with PTC. AXIN1 rs1805105 G/A polymorphism was associated with higher tumor size.

Keywords: AXIN1; CTSB; papillary thyroid cancer; polymorphism; tumor size.

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Conflict of interest statement

All the authors declare no conflict of interest to disclose.

Figures

FIGURE 1
FIGURE 1
Agarose gel electrophoresis images of polymerase chain reaction restriction fragments length polymorphism (PCR–RFLP) for (A) AXIN1 rs1805105, (B) AXIN1 rs12921862, and (C) CTSB rs12898 polymorphisms.
See this image and copyright information in PMC

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