Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2557:645-659.
doi: 10.1007/978-1-0716-2639-9_39.

Targeted Protein Unfolding at the Golgi Apparatus

Jasmin Schillinger  1 , Doris Hellerschmied  2
Affiliations

Targeted Protein Unfolding at the Golgi Apparatus

Jasmin Schillinger et al. Methods Mol Biol. 2023.

Abstract

Maintaining protein homeostasis (proteostasis) is vital to cellular and organismal health. How the Golgi apparatus, the central protein maturation and sorting station in the cell, manages misfolded proteins to maintain proteostasis is still poorly understood. Here we present a strategy for targeted protein unfolding at the Golgi that enables studying Golgi-related protein quality control and stress-signaling pathways. Targeted protein unfolding is induced by small molecule-based chemical biology approaches-hydrophobic tagging and the use of a destabilization domain. Imaging studies allow visualizing quality control (QC) phenotypes, such as the formation of QC carriers and Golgi-to-endoplasmic reticulum trafficking, and correlating these phenotypes with other trafficking processes.

Keywords: Chemical biology tools; Destabilization domain; HaloTag fusion proteins; Hydrophobic tagging; SNAP-TMR pulse labeling; Targeted protein unfolding; Trimethoprim.

PubMed Disclaimer

References

    1. De Matteis MA, Luini A (2008) Exiting the Golgi complex. Nat Rev Mol Cell Biol 9(4):273–284. https://doi.org/10.1038/nrm2378 - DOI
    1. Ramazanov BR, Tran ML, von Blume J (2021) Sending out molecules from the TGN. Curr Opin Cell Biol 71:55–62. https://doi.org/10.1016/j.ceb.2021.02.005 - DOI
    1. James Morre D, Mollenhauer HH (2007) Microscopic morphology and the origins of the membrane maturation model of Golgi apparatus function. Int Rev Cytol 262:191–218. https://doi.org/10.1016/S0074-7696(07)62004-X - DOI
    1. Lippincott-Schwartz J, Phair RD (2010) Lipids and cholesterol as regulators of traffic in the endomembrane system. Annu Rev Biophys 39:559–578. https://doi.org/10.1146/annurev.biophys.093008.131357 - DOI
    1. Barlowe CK, Miller EA (2013) Secretory protein biogenesis and traffic in the early secretory pathway. Genetics 193(2):383–410. https://doi.org/10.1534/genetics.112.142810 - DOI

Publication types

MeSH terms

LinkOut - more resources