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. 2022 Aug 17;10(12):4226-4237.
doi: 10.1002/fsn3.3016. eCollection 2022 Dec.

The effects of biogenic amines in Chinese Huangjiu on the behavior of mice and hangover headache-related indices

Wenmei Zhao  1   2 , Zukang Fu  3 , Xin Wang  4 , Qingzhong Mao  3 , Chunguang Luan  2 , Shanbin Chen  2 , Fengjie Zhang  2 , Jiajun Yu  2 , Yiping Yao  2 , Yishu Li  2 , Feike Hao  2 , Deliang Wang  2 , Nan Li  1 , Jie Huangfu  2 , Chengtao Wang  4
Affiliations

The effects of biogenic amines in Chinese Huangjiu on the behavior of mice and hangover headache-related indices

Wenmei Zhao et al. Food Sci Nutr. .

Abstract

Huangjiu (Chinese rice wine) is a popular and traditional alcoholic beverage in China; however, the consumption of Huangjiu readily results in hangover symptoms. The aim of this study was to identify the main components associated with behavioral inhibition, headache, and the relevant mechanisms by using a mice hangover model. The results of an open-field experiment revealed that the key biogenic amine associated with mice behavior was histamine, which inhibited the behavior activity of mice in a dose-dependent manner. Moreover, histamine treatment decreased the levels of serotonin (5-HT) and 5-hydroxyindole acetic acid. In addition, the levels of dopamine and nitric oxide, which are associated with migraine, increased in the brain tissue of mice. In addition, the expression of receptor genes of 5-HT, including Htr1a, Htr1f, and Htr2c, is essential in regulating various behaviors and mental activities. In conclusion, the present study demonstrated that histamine is a key component in Huangjiu, and it is related to hangover symptoms by affecting the level of 5-HT and its receptors.

Keywords: 5‐HT; Huangjiu; biogenic amines; headache; histamine.

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Conflict of interest statement

The authors have declared that there is no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Influence of BAs on mice behavior in treatment groups. (a) Moving distance of mice. (b) Average speed of mice. (c) Moving time of mice. (d) Stagnation time of mice. (e) Number of stagnation periods of mice. BA, biogenic amines; CAD, cadaverine; H, high; HIM, histamine; L, low; PEA, 2‐phenylethylamine; PUT, putrescine; TYM, tyramine. Values are expressed as mean ± SD (n = 10). *p < .05 and **p < .01 versus control group.
FIGURE 2
FIGURE 2
Concentration of histamine in four wine samples and the effects of different Huangjiu samples on mice behavior. (a) The concentration of histamine in four wine samples, (b) Moving distance, (c) average speed, (d) moving time, (e) stagnation time, and (f) number of stagnation periods. Values are expressed as mean ± SD (n = 10). *p < .05 and **p < .01 versus control wine group.
FIGURE 3
FIGURE 3
Effect of histamine concentration on mice behavior. (a) Moving distance, (b) average speed, (c) moving time, (d) stagnation time, and (e) number of stagnation periods. H, high; L, low; and M, middle. Values are expressed as mean ± SD (n = 10). *p < .05 and **p < .01 versus control group.
FIGURE 4
FIGURE 4
Content of 5‐HT, 5‐HIAA, DA, and NO in brain tissue. (a) The concentration of 5‐HT in mouse brain tissue (n = 10). (b) The concentration of 5‐HIAA in mouse brain tissue. (c) The concentration of DA in mouse brain tissue. (d) The concentration of NO in mouse brain tissue. Values are expressed as mean ± SD (n = 10). *p < .05 versus, **p < .01 versus control group.
FIGURE 5
FIGURE 5
Effects of histamine on the relative mRNA expression. (a) Expression of Htr1a. (b) Expression of Htr1f. (c) Expression of Htr2c. Values are expressed as mean ± SD (n = 10). *p < .05 versus control group, **p < .01 versus control group.
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