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. 2022 Mar 31;18(3):289-292.
doi: 10.6026/97320630018289. eCollection 2022.

Effect of Carica papaya on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro

Mohamed Rifaath  1 , Preetha Santhakumar  1 , J Selvaraj  1
Affiliations

Effect of Carica papaya on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro

Mohamed Rifaath et al. Bioinformation. .

Abstract

Colon cancer is the third most frequent cancer in humans. Carica papaya leaves are vegetable foods consumed by most people around the world; it has potential as an anticancer. Therefore it is of interest to investigate the effect of Carica papaya on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro. Human Colon cancer cell line (HT-29) was purchased from the National Centre for Cell Sciences, Pune, India. Cell viability test was done by MTT assay. Gene expression analysis was done by Real Time-PCR. The obtained data were analyzed statistically by one-way analysis of variance and Duncan's multiple range test with Graph Pad Prism version 5 to analyze the significance of individual variations among the control and experimental groups. The significance was considered at p<0.05 level in Duncan's test. Carica papaya caused a marked increase in cell death in a dose dependent manner. At the end of 48 hours, maximum inhibition was at 300 and 400 µg/ml. Carica papaya has significantly reduced the mRNA expression of Wnt and beta catenin (p<0.05). Data showed that Carica papaya leaf extract has anticancer activity on Colon cancer cell lines (HT-29).

Keywords: Carica papaya; Wnt; beta catenin; human colon cancer (HT-29) cells; in vitro; mRNA expression.

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Conflict of interest statement

Nil

References

    1. Preetha S, et al. International Journal of Pharmaceutical Research . 2020
    2. {'text': '', 'ref_index': 2, 'ids': [{'type': 'DOI', 'value': '10.31838/ijpr/2020.sp1.450', 'is_inner': False, 'url': 'https://doi.org/10.31838/ijpr/2020.sp1.450'}]}
    1. Preetha S, et al. International Journal of Pharmaceutical Research . 2020
    2. {'text': '', 'ref_index': 2, 'ids': [{'type': 'DOI', 'value': '10.31838/ijpr/2020.sp1.438', 'is_inner': False, 'url': 'https://doi.org/10.31838/ijpr/2020.sp1.438'}]}
    1. Anushya PA, et al. International Journal of Research in Pharmaceutical Sciences . 2021;12:6.
    2. {'text': '', 'ref_index': 2, 'ids': [{'type': 'DOI', 'value': '10.26452/ijrps.v12i1.3905', 'is_inner': False, 'url': 'https://doi.org/10.26452/ijrps.v12i1.3905'}]}
    1. Chockalingam S, et al. Journal of Evolution of Medical and Dental Sciences . 2020;9:2947.
    2. {'text': '', 'ref_index': 2, 'ids': [{'type': 'DOI', 'value': '10.14260/jemds/2020/646', 'is_inner': False, 'url': 'https://doi.org/10.14260/jemds/2020/646'}]}
    1. Janani KJ, et al. International Journal of Research in Pharmaceutical Sciences . 2020;11:6211.
    2. {'text': '', 'ref_index': 2, 'ids': [{'type': 'DOI', 'value': '10.26452/ijrps.v11i4.3298', 'is_inner': False, 'url': 'https://doi.org/10.26452/ijrps.v11i4.3298'}]}

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