Reply to: Assessment of 3D MINFLUX data for quantitative structural biology in cells

Affiliations

¹ Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
² Research Institute of Molecular Pathology, Vienna, Austria.
³ Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
⁴ Abberior Instruments GmbH, Göttingen, Germany.
⁵ Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany. stefan.hell@mpinat.mpg.de.
⁶ Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Heidelberg, Germany. stefan.hell@mpinat.mpg.de.

Comment

Klaus C Gwosch et al. Nat Methods. 2023 Jan.

. 2023 Jan;20(1):52-54.

doi: 10.1038/s41592-022-01695-w. Epub 2022 Dec 15.

¹ Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
² Research Institute of Molecular Pathology, Vienna, Austria.
³ Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
⁴ Abberior Instruments GmbH, Göttingen, Germany.
⁵ Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany. stefan.hell@mpinat.mpg.de.
⁶ Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Heidelberg, Germany. stefan.hell@mpinat.mpg.de.

No abstract available

Comment on

MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells.
Gwosch KC, Pape JK, Balzarotti F, Hoess P, Ellenberg J, Ries J, Hell SW. Gwosch KC, et al. Nat Methods. 2020 Feb;17(2):217-224. doi: 10.1038/s41592-019-0688-0. Epub 2020 Jan 13. Nat Methods. 2020. PMID: 31932776
Assessment of 3D MINFLUX data for quantitative structural biology in cells.
Prakash K, Curd AP. Prakash K, et al. Nat Methods. 2023 Jan;20(1):48-51. doi: 10.1038/s41592-022-01694-x. Epub 2022 Dec 15. Nat Methods. 2023. PMID: 36522506 No abstract available.

1. Prakash, K. & Curd, A. P. Assessment of 3D MINFLUX data for quantitative structural biology in cells. Nat. Methods https://doi.org/10.1038/s41592-022-01694-x (2022).
1. Gwosch, K. C. et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat. Methods 17, 217–224 (2020). - DOI
1. Balzarotti, F. et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355, 606–612 (2017). - DOI
1. Sabinina, V. J. et al. Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex. Mol. Biol. Cell 32, 1523–1533 (2021). - DOI
1. Löschberger, A., Franke, C., Krohne, G., van de Linde, S. & Sauer, M. Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution. J. Cell Sci. 127, 4351–4355 (2014).