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. 2023 Aug 4;24(Suppl 1):S71-S80.
doi: 10.1093/pm/pnac197.

Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program

Aaron J Fields  1 , Stefan Dudli  2 , Andrew Schrepf  3 , Angie Kim  4 , Bernice Pham  4 , Estefania Gallego  4 , Sandra Mendoza  4 , Sharon B Meropol  5 , Jessa Darwin  6 , Gwendolyn Sowa  6   7 , Nam V Vo  7   8
Affiliations

Protocol for Biospecimen Collection and Analysis Within the BACPAC Research Program

Aaron J Fields et al. Pain Med. .

Abstract

The Biospecimen Collection and Processing Working Group of the National Institutes of Health (NIH) HEAL Initiative BACPAC Research Program was charged with identifying molecular biomarkers of interest to chronic low back pain (cLBP). Having identified biomarkers of interest, the Working Group worked with the New York University Grossman School of Medicine, Center for Biospecimen Research and Development-funded by the Early Phase Pain Investigation Clinical Network Data Coordinating Center-to harmonize consortium-wide and site-specific efforts for biospecimen collection and analysis. Biospecimen collected are saliva, blood (whole, plasma, serum), urine, stool, and spine tissue (paraspinal muscle, ligamentum flavum, vertebral bone, facet cartilage, disc endplate, annulus fibrosus, or nucleus pulposus). The omics data acquisition and analyses derived from the biospecimen include genomics and epigenetics from DNA, proteomics from protein, transcriptomics from RNA, and microbiomics from 16S rRNA. These analyses contribute to the overarching goal of BACPAC to phenotype cLBP and will guide future efforts for precision medicine treatment.

Keywords: Biomarkers; Biospecimen; Chronic Low Back Pain; Omics.

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Figures

Figure 1.
Figure 1.
Summary schematic of biospecimen and omics analyses.
See this image and copyright information in PMC

References

    1. Borenstein D. Epidemiology, etiology, diagnostic evaluation, and treatment of low back pain. Current Opin Rheumatol 1992;4(2):226–32. - PubMed
    1. Borenstein DG. Chronic low back pain. Rheum Dis Clin North Am 1996;22(3):439–56. doi:10.1016/s0889-857x(05)70281-7. - DOI - PubMed
    1. National Institute of Neurological Disorders and Stroke. Back Pain Fact Sheet (NIH Publication No. 20-NS-5161). 2020. Available at: https://www.ninds.nih.gov/health-information/patient-caregiver-education... (accessed 3 January 2023).
    1. Allegri M, De Gregori M, Minella CE, et al.; PainOMICS Group. ‘Omics’ biomarkers associated with chronic low back pain: Protocol of a retrospective longitudinal study. BMJ Open 2016;6(10):e012070. doi:10.1136/bmjopen-2016-012070. - DOI - PMC - PubMed
    1. Dagostino C, De Gregori M, Gieger C, et al.; PainOmics Group. Validation of standard operating procedures in a multicenter retrospective study to identify -omics biomarkers for chronic low back pain. PLoS One 2017;12(5):e0176372. doi:10.1371/journal.pone.0176372 - DOI - PMC - PubMed

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