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. 2023 Aug;26(3):471-474.
doi: 10.1007/s10123-022-00311-3. Epub 2022 Dec 17.

High performance of the automated ADVIA Centaur Systems SARS-CoV-2 Antigen Assay in nasopharyngeal samples with high viral load

Esther Ríos  1   2 , Sara Medrano  3 , Mar Alvarez  3 , María José Valderrama  4 , Luis Vallejo  3 , Alberto Delgado-Iribarren  5   3 , Esther Culebras  5   3
Affiliations

High performance of the automated ADVIA Centaur Systems SARS-CoV-2 Antigen Assay in nasopharyngeal samples with high viral load

Esther Ríos et al. Int Microbiol. 2023 Aug.

Abstract

ADVIA Centaur SARS-CoV-2 Antigen (COV2Ag) Assay (Siemens Healthineers) was evaluated for SARS-CoV-2 detection. A total of 141 nasopharyngeal samples were analyzed by this technique and results were compared with those obtained by quantitative reverse-transcription polymerase chain reaction (RT-PCR). The overall sensitivity and specificity of the test were 68.70% and 70%, respectively. Regarding cycle threshold (Ct) values, the COV2Ag test showed a sensitivity of 93.75% and 100% for nasopharyngeal samples with Ct < 25 and < 20, respectively. ADVIA Centaur COV2Ag Assay is a useful, automated, and rapid technique for early SARS-CoV-2 diagnosis and isolation of the infected individuals, avoiding its transmission.

Keywords: PCR; Rapid antigen test; SARS-CoV-2; Sensitivity; Specificity.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
SARS-CoV-2 antigen detection according to RT-PCR results. a Distribution of CoVAg index values according to RT-PCR results. b Distribution of CoVAg index values according to Ct values. Data are presented as mean ± SEM
See this image and copyright information in PMC

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