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. 1987 Jul;6(7):2003-10.
doi: 10.1002/j.1460-2075.1987.tb02464.x.

Periodic DNA synthesis in cell-free extracts of Xenopus eggs

C J Hutchison  1 , R Cox, R S Drepaul, M Gomperts, C C Ford
Affiliations

Periodic DNA synthesis in cell-free extracts of Xenopus eggs

C J Hutchison et al. EMBO J. 1987 Jul.

Abstract

Cell-free extracts prepared from unfertilized eggs of Xenopus laevis support DNA synthesis on sperm pronuclei. Continuous labelling studies using [3H]dCTP and pulse labelling studies using [32P]dCTP demonstrate that synthesis occurs in short bursts of 40 min, which are punctuated by periods of 20-40 min during which no synthesis occurs. Density substitution experiments using bromodeoxyuridine demonstrate that this synthesis involves the initiation of replication and reveals that re-initiation events can occur following multiple bursts of replication. The periodic properties of these extracts are sensitive to protein synthesis inhibitors.

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