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. 2022 Nov 29;8(12):1260.
doi: 10.3390/jof8121260.

Isolation and Characterization of Ruminal Yeast Strain with Probiotic Potential and Its Effects on Growth Performance, Nutrients Digestibility, Rumen Fermentation and Microbiota of Hu Sheep

Yao Wang  1   2 , Zihao Li  1   2 , Wei Jin  1   2 , Shengyong Mao  1   2
Affiliations

Isolation and Characterization of Ruminal Yeast Strain with Probiotic Potential and Its Effects on Growth Performance, Nutrients Digestibility, Rumen Fermentation and Microbiota of Hu Sheep

Yao Wang et al. J Fungi (Basel). .

Abstract

Yeast strains are widely used in ruminant production. However, knowledge about the effects of rumen native yeasts on ruminants is limited. Therefore, this study aimed to obtain a rumen native yeast isolate and investigate its effects on growth performance, nutrient digestibility, rumen fermentation and microbiota in Hu sheep. Yeasts were isolated by picking up colonies from agar plates, and identified by sequencing the ITS sequences. One isolate belonging to Pichia kudriavzevii had the highest optical density among these isolates obtained. This isolate was prepared to perform an animal feeding trial. A randomized block design was used for the animal trial. Sixteen Hu sheep were randomly assigned to the control (CON, fed basal diet, n = 8) and treatment group (LPK, fed basal diet plus P. kudriavzevii, CFU = 8 × 109 head/d, n = 8). Sheep were housed individually and treated for 4 weeks. Compared to CON, LPK increased final body weight, nutrient digestibility and rumen acetate concentration and acetate-to-propionate ratio in sheep. The results of Illumina MiSeq PE 300 sequencing showed that LPK increased the relative abundance of lipolytic bacteria (Anaerovibrio spp. and Pseudomonas spp.) and probiotic bacteria (Faecalibacterium spp. and Bifidobacterium spp.). For rumen eukaryotes, LPK increased the genera associated with fiber degradation, including protozoan Polyplastron and fungus Pichia. Our results discovered that rumen native yeast isolate P. kudriavzevii might promote the digestion of fibers and lipids by modulating specific microbial populations with enhancing acetate-type fermentation.

Keywords: Pichia kudriavzevii; fiber degradation; microbial community composition; rumen fermentation; rumen native yeast.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Growth curves of 14 rumen-derived yeast strains incubated for 48 h at 39 °C and 150 rmp in a culture-shaker under aerobic conditions.
Figure 2
Figure 2
Principal coordinate analysis (PCoA) of rumen bacteria (A), protozoa (B), and fungi (C) based on operational taxon data. Control (CON) group (blue circle), live Pichia kudriavzevii (LPK) group (red circle).
Figure 3
Figure 3
The bacterial community in the rumen of Hu sheep: (A) The mean relative abundance of phylum ≥ 0.5% in at least one group is present. (B) The mean relative abundance of genus ≥ 0.5% in at least one group is present. Error bars, SEM. *, p < 0.05. CON = control group; LPK = live Pichia kudriavzevii group.
Figure 4
Figure 4
The rumen protozoa community in Hu sheep: (A) The mean relative abundance of phylum ≥ 1% in at least one group is present. (B) The mean relative abundance of genus ≥ 1% in at least one group is present. Error bars, SEM. * p < 0.05. CON = control group; LPK = live Pichia kudriavzevii group.
Figure 5
Figure 5
The rumen fungal community in Hu sheep: (A) The mean relative abundance of phylum ≥ 1% in at least one group is present. (B) The mean relative abundance of genus ≥ 1% in at least one group is present. Error bars, SEM. * p < 0.05. CON = control group; LPK = live Pichia kudriavzevii group.
Figure 6
Figure 6
Spearman rank correlation analysis between rumen microbial community (top 10 relative abundance at the genus level of bacteria, protozoa and fungi), Pichia kudriavzevii and rumen volatile acids. Red, positive; blue, negative. * p < 0.05.
See this image and copyright information in PMC

References

    1. Weimer P.J. Redundancy, Resilience, and Host Specificity of the Ruminal Microbiota: Implications for Engineering Improved Ruminal Fermentations. Front. Microbiol. 2015;6:296. doi: 10.3389/fmicb.2015.00296. - DOI - PMC - PubMed
    1. Wang Y.-L., Wang W.-K., Wu Q.-C., Yang H.-J. The Release and Catabolism of Ferulic Acid in Plant Cell Wall by Rumen Microbes: A Review. Anim. Nutr. 2022;9:335–344. doi: 10.1016/j.aninu.2022.02.003. - DOI - PMC - PubMed
    1. Fonty G., Chaucheyras-Durand F. Effects and Modes of Action of Live Yeasts in the Rumen. Biologia. 2006;61:741–750. doi: 10.2478/s11756-006-0151-4. - DOI
    1. Chaucheyras-Durand F., Walker N.D., Bach A. Effects of Active Dry Yeasts on the Rumen Microbial Ecosystem: Past, Present and Future. Anim. Feed Sci. Technol. 2008;145:5–26. doi: 10.1016/j.anifeedsci.2007.04.019. - DOI
    1. Moya D., Ferret A., Blanch M., Fuentes M.C., Fandiño J.I., Calsamiglia S. Effects of Live Yeast (Saccharomyces cerevisiae) and Type of Cereal on Rumen Microbial Fermentation in a Dual Flow Continuous Culture Fermentation System. J. Anim. Physiol. Anim. Nutr. 2018;102:1488–1496. doi: 10.1111/jpn.12975. - DOI - PubMed

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