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. 1987 Sep;25(9):1766-8.
doi: 10.1128/jcm.25.9.1766-1768.1987.

Determination of hippurate hydrolysis by gas-liquid chromatography

P L Wallace  1 , C M Patton, C W Moss
Affiliations

Determination of hippurate hydrolysis by gas-liquid chromatography

P L Wallace et al. J Clin Microbiol. 1987 Sep.

Abstract

A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37 degrees C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive, and its specificity provides a high degree of reliability for organisms with weak hippuricase activity.

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References

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