Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec 16;11(24):4078.
doi: 10.3390/foods11244078.

Rapid Screening of High-Yield Gellan Gum Mutants of Sphingomonas paucimobilis ATCC 31461 by Combining Atmospheric and Room Temperature Plasma Mutation with Near-Infrared Spectroscopy Monitoring

Ling Sun  1 , Yazhen Wang  1 , Meixiang Yue  1 , Xialiang Ding  1 , Xiangyang Yu  2   3 , Jing Ge  2   3 , Wenjing Sun  1 , Lixiao Song  2   3
Affiliations

Rapid Screening of High-Yield Gellan Gum Mutants of Sphingomonas paucimobilis ATCC 31461 by Combining Atmospheric and Room Temperature Plasma Mutation with Near-Infrared Spectroscopy Monitoring

Ling Sun et al. Foods. .

Abstract

In this study, an efficient mutagenesis and rapid screening method of high-yield gellan gum mutant by atmospheric and room temperature plasma (ARTP) treatment combined with Near-Infrared Spectroscopy (NIRS) was proposed. A NIRS model for the on-line detection of gellan gum yield was constructed by joint interval partial least squares (siPLS) regression on the basis of chemical determination and NIRS acquisition of gellan gum yield. Five genetically stable mutant strains were screened using the on-line NIRS detection of gellan gum yield in the fermentation from approximately 600 mutant strains induced by ARTP. Remarkably, compared with the original strain, the gellan gum yield of mutant strain 519 was 9.427 g/L (increased by 133.5%) under the optimal fermentation conditions, which was determined by single-factor and response surface optimization. Therefore, the method of ARTP mutation combined with the NIRS model can be used to screen high-yield mutant strains of gellan gum and other high-yield polysaccharide strains.

Keywords: Near Infrared Spectroscopy; Sphingomonas paucimobilis; atmospheric and room temperature plasma (ARTP); fast screening; gellan gum.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The growth curve (A) and the mortality rate treated by atmospheric and room temperature plasma (ARTP) (B) of S. paucimobilis ATCC 31461. The arrow in (A) indicates the optimal culture time point for ARTP mutation.
Figure 2
Figure 2
Near Infrared Spectroscopy (NIRS) acquisition and model construction of gellan gum yield. (A) Original spectrogram; (B) Best subinterval; (C) Training set; and (D) Prediction set.
Figure 3
Figure 3
The predicted yield of gellan gum from mutant strains induced by ARTP. (A) the mutant strain 1–128; (B) the mutant strain 129–230; (C) the mutant strain 238–276, 415–459; (D) the mutant strain 460–531.
Figure 4
Figure 4
Genetic stability of the high-yield gellan gum mutants within ten generations. Different letters represent a significant difference between the two in a strain (p < 0.05).
Figure 5
Figure 5
Optimization of fermentation conditions of mutant strain 519 by single-factor experiments. (A) Carbon sources; (B) Glucose content; (C) Nitrogen sources; (D) Soybean meal content; (E) Inoculum concentration; (F) Initial pH; (G) Culture time in seed solution; and (H) Percentage of fermentation liquid in bottle. Different letters represent a significant difference between the two in a strain (p < 0.05).
Figure 6
Figure 6
Optimization of fermentation conditions of mutant by Response Surface Methodology. (A) The effect of the interaction of fermentation conditions on the yield of gellan gum; (B) The experimental verification of the optimal fermentation conditions and yield. “**” represents a significant difference (p < 0.01).
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Fialho A.M., Martins L.O., Donval M.L., Leitao J.H., Ridout M.J., Jay A.J., Morris V.J., Sa-Correia I.I. Structures and properties of gellan polymers produced by Sphingomonas paucimobilis ATCC 31461 from lactose compared with those produced from glucose and from cheese whey. Appl. Environ. Microbiol. 1999;65:2485–2491. doi: 10.1128/AEM.65.6.2485-2491.1999. - DOI - PMC - PubMed
    1. Gai Z., Wang X., Zhang X., Su F., Wang X., Tang H., Tai C., Tao F., Ma C., Xu P. Genome sequence of Sphingomonas elodea ATCC 31461, a highly productive industrial strain of gellan gum. J. Bacteriol. 2011;193:7015–7016. doi: 10.1128/JB.06307-11. - DOI - PMC - PubMed
    1. Jansson P.E., Lindberg B., Sandford P.A. Structural studies of gellan gum, an extracellular polysaccharide elaborated by Pseudomonas elodea. Carbohydr. Res. 1983;124:135–139. doi: 10.1016/0008-6215(83)88361-X. - DOI
    1. McMaster L.D., Kokott S.A., Reid S.J., Abratt V.R. Use of traditional African fermented beverages as delivery vehicles for Bifidobacterium lactis DSM 10140. Int. J. Food Microbiol. 2005;102:231–237. doi: 10.1016/j.ijfoodmicro.2004.12.013. - DOI - PubMed
    1. Sun W.R., Griffiths M.W. Survival of bifidobacteria in yogurt and simulated gastric juice following immobilization in gellan–xanthan beads. Int. J. Food Microbiol. 2000;61:17–25. doi: 10.1016/S0168-1605(00)00327-5. - DOI - PubMed

LinkOut - more resources