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. 2022 Dec 10;23(24):15669.
doi: 10.3390/ijms232415669.

Plasma and Peritoneal Fluid Fibronectin and Collagen IV Levels as Potential Biomarkers of Endometriosis

Damian Warzecha  1 , Julia Załęcka  1 , Grzegorz Mańka  2 , Mariusz Kiecka  2 , Michał Lipa  1 , Robert Spaczyński  3 , Piotr Piekarski  3 , Beata Banaszewska  3 , Artur Jakimiuk  4   5 , Tadeusz Issat  4   5 , Wojciech Rokita  6   7 , Jakub Młodawski  6   7 , Maria Szubert  8   9 , Piotr Sieroszewski  8   10 , Grzegorz Raba  11   12 , Kamil Szczupak  11   12 , Tomasz Kluz  13 , Marek Kluza  13 , Mirosław Wielgoś  1 , Łukasz Ołdak  14 , Anna Leśniewska  14 , Ewa Gorodkiewicz  14 , Piotr Laudański  1   15   16   17
Affiliations

Plasma and Peritoneal Fluid Fibronectin and Collagen IV Levels as Potential Biomarkers of Endometriosis

Damian Warzecha et al. Int J Mol Sci. .

Abstract

Laparoscopy as a diagnostic tool for patients with suspected endometriosis is associated with several potentially life-threatening complications. Therefore, it is imperative to identify reliable, non-invasive biomarkers of the disease. The aim of this study was to analyse the concentrations of fibronectin and type IV collagen in peritoneal fluid and plasma to assess their role as potential biomarkers in the diagnosis of endometriosis. Fibronectin and collagen IV protein levels were assessed by surface plasmon resonance imaging (SPRi) biosensors with the usage of monoclonal antibodies. All patients enrolled in the study were referred for laparoscopy for the diagnosis of infertility or chronic pelvic pain (n = 84). The study group included patients with endometriosis confirmed during surgery (n = 49). The concentration of fibronectin in the plasma (329.3 ± 98.5 mg/L) and peritoneal fluid (26.8 ± 11.1 μg/L) in women with endometriosis was significantly higher than in the control group (251.2 ± 84.0 mg/L, 7.0 ± 5.9 μg/L). Fibronectin levels were independent of endometriosis stage (p = 0.874, p = 0.469). No significant differences were observed in collagen IV levels (p = 0.385, p = 0.465). The presence of elevated levels of fibronectin may indicate abnormalities in cell-ECM signalling during the course of endometriosis, and may be a potential biomarker for early detection.

Keywords: collagen IV; endometriosis; fibronectin; peritoneal fluid; plasma.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Logistic regression for endometriosis OR and 95% CI (n = 59).
Figure 2
Figure 2
The ROC curve analysis for diagnostic efficacy of fibronectin in peritoneal fluid for endometriosis.
Figure 3
Figure 3
The ROC curve analysis for diagnostic efficacy of fibronectin in plasma for endometriosis.
Figure 4
Figure 4
The ROC curve analysis for diagnostic efficacy of both fibronectin in plasma and peritoneal fluid for endometriosis.
See this image and copyright information in PMC

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