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. 2022 Dec 15;58(12):1842.
doi: 10.3390/medicina58121842.

Platelet-Rich Plasma for Pleurodesis: An Experimental Study in Rabbits

Styliani Maria Kolokotroni  1   2 , Dimitrios Lamprinos  3 , Nikolaos Goutas  4 , Emmanouil I Kapetanakis  5 , Konstantinos Kontzoglou  2   6 , Despoina Perrea  2 , Periklis Tomos  5
Affiliations

Platelet-Rich Plasma for Pleurodesis: An Experimental Study in Rabbits

Styliani Maria Kolokotroni et al. Medicina (Kaunas). .

Abstract

Background and Objectives: This study was designed to evaluate platelet-rich plasma (PRP) as a method of pleurodesis in a rabbit model. Pleurodesis with PRP was compared against the gold-standard use of talc. The secondary evaluation assessed the ideal time for achieving pleurodesis. Materials and Methods: 25 healthy New Zealand white rabbits were assigned to three groups, as follows: 12 animals in the first and second groups, as well as one animal with no intervention in the final group, which was used as a control. The talc pleurodesis group (baseline) underwent pleurodesis with sterile talc, which is the gold-standard sclerosing agent used for pleurodesis. The PRP group underwent pleurodesis using autologous PRP. The last group had one rabbit with no intervention. A total of 12 rabbits (n = 6 for the talc pleurodesis group and n = 6 for the PRP group) were sacrificed 3 days (72 h) after the intervention, and 12 rabbits (n = 6 for the talc pleurodesis group and n = 6 for the PRP group) were sacrificed 6 days (144 h) after the intervention. In both the talc and PRP group, FBC and CRP were measured before the intervention and in 3 or 6 days afterwards, respectively. The pleura and the lungs were evaluated histopathologically. Results: Macroscopically, there were no statistically significant differences between the two groups. In terms of microscopic findings, there were no statistically significant differences in inflammatory reactions provoked in the visceral and parietal pleura between the PRP and talc. In addition, with talc pleurodesis, a foreign-body reaction was observed in about 50% of the cases, which was not observed with PRP. In terms of inflammation between 3 and 6 days, there were no statistically significant differences with PRP, there was only a statistically significant difference between 3 and 6 days regarding the parietal pleura in the talc group. Conclusions: The instillation of autologous PRP in the pleural cavity shows promise in achieving pleurodesis. The efficacy of PRP as a pleurodesis agent should be examined further.

Keywords: PRP; pleurodesis; rabbits; talc.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
×100 Hematoxylin-Eosin stain. Foreign Body Crystal Aggregations after talc installation and Pulmonary Parenchymal Thickening.
Figure 2
Figure 2
×40 Masson-Trichrome stain: Following talc installation, fibrosis is present under the mesothelial layer.
Figure 3
Figure 3
×100 Hematoxylin-Eosin stain—after PRP installation, there is significant fibrosis peripherally.
Figure 4
Figure 4
×200 Masson-Trichrome stain: Following PRP installation, fibrosis is present under the mesothelial layer.
Figure 5
Figure 5
Visceral pleura inflammation of PRP vs. talc.
Figure 6
Figure 6
Parietal pleura inflammation of PRP vs. talc.
Figure 7
Figure 7
Visceral pleura inflammation at 3 vs. 6 days of PRP and talc combined.
Figure 8
Figure 8
Parietal pleura inflammation at 3 vs. 6 days of PRP and talc combined.
Figure 9
Figure 9
Visceral pleura inflammation 3 vs. 6 days of PRP.
Figure 10
Figure 10
Parietal pleura inflammation 3 vs. 6 days of PRP.
Figure 11
Figure 11
Parietal pleura inflammation at 3 vs. 6 days of talc.
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