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Review
. 2022 Nov 24;10(12):2327.
doi: 10.3390/microorganisms10122327.

Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome

Nurlan Sandybayev  1 , Vyacheslav Beloussov  1   2 , Vitaliy Strochkov  1 , Maxim Solomadin  3 , Joanna Granica  2 , Sergey Yegorov  4
Affiliations
Review

Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome

Nurlan Sandybayev et al. Microorganisms. .

Abstract

The COVID-19 pandemic and heightened perception of the risk of emerging viral infections have boosted the efforts to better understand the virome or complete repertoire of viruses in health and disease, with a focus on infectious respiratory diseases. Next-generation sequencing (NGS) is widely used to study microorganisms, allowing the elucidation of bacteria and viruses inhabiting different body systems and identifying new pathogens. However, NGS studies suffer from a lack of standardization, in particular, due to various methodological approaches and no single format for processing the results. Here, we review the main methodological approaches and key stages for studies of the human virome, with an emphasis on virome changes during acute respiratory viral infection, with applications for clinical diagnostics and epidemiologic analyses.

Keywords: ARVI; DNA/RNA; NGS; acute respiratory infections; metagenomics; virome; virus.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Most of the ways of transmission respiratory viruses.
Figure 2
Figure 2
General scheme for NGS sequencing of respiratory viruses.
See this image and copyright information in PMC

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