Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium
- PMID: 36557584
- PMCID: PMC9781318
- DOI: 10.3390/microorganisms10122331
Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium
Abstract
A bacterial consortium capable of degrading ethyl tert-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. Arthrobacter sp., Herbaspirillum sp., Pseudacidovorax sp., Pseudomonas sp., and Xanthomonas sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). Xanthomonas sp. and Pseudomonas sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.
Keywords: 2D-DIGE; ETBE; bacterial consortium; biodegradation; metaproteome.
Conflict of interest statement
All the authors state that they have no conflict of interest.
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References
-
- Barceló D., Arvin E. Fuel Oxygenates. Springer; Berlin/Heidelberg, Germany: 2007.
-
- Kian F.Y., Abdul R.M., Soon H.T. A review on the evolution of ethyl tert-butyl ether (ETBE) and its future prospects. Renew. Sustain. Energy Rev. 2013;22:604–620.
-
- Squillace P.J., Zogorski J.S., Wilber W.G., Price C.V. Preliminary Assessment of the Occurrence and Possible Sources of MTBE in Groundwater in the United States. 1993−1994. Environ. Sci. Technol. 1996;30:1721–1730. doi: 10.1021/es9507170. - DOI
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