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. 2022 Dec 7;10(12):2420.
doi: 10.3390/microorganisms10122420.

The Colombian Zika Virus Isolate (COL345Si) Replicates in Prostate Adenocarcinoma Cells and Modulates the Antiviral Response

Yaneth Miranda Brand  1   2 , Astrid M Bedoya  3 , Liliana Betancur-Galvis  3 , Juan Carlos Gallego-Gómez  2
Affiliations

The Colombian Zika Virus Isolate (COL345Si) Replicates in Prostate Adenocarcinoma Cells and Modulates the Antiviral Response

Yaneth Miranda Brand et al. Microorganisms. .

Abstract

Zika virus (ZIKV), a flavivirus that is mainly transmitted by A. aegypti and A. albopictus and sexual transmission, has been documented and described. The ZIKV RNA detection in the semen of vasectomized men indicates that accessory glands such as the prostate could be a site of virus replication. In this study, we characterized the ZIKV infection, evaluated the antiviral profile, and demonstrated the AXL and TIM-1 expression on the PC3 prostate cell line. It was also determined that PC3 cells are susceptible and permissive to ZIKV infection without altering the cell viability or causing a cytopathic effect. The antiviral profile suggests that the PC3 cells modulate the antiviral response through the suppressor molecule expression, SOCS-1, during a ZIKV infection.

Keywords: ZIKV; antiviral response; flavivirus; permissive infection; prostate.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Phylogenetic tree of several strains of the Zika virus in comparison with the Zika isolated COL354Si GenBank: MH179341.1 (indicated with a blue point) showed similar mutations to the other Asiatic strains.
Figure 2
Figure 2
PC3 and Vero E6 cells express AXL and TIM-1 cell receptors. Pictures were captured at a 60× magnification. The photographs represent the subcellular location of AXL (b,f) and TIM-1 (d,h), and they correspond to three independent triplicate experiments. AXL and TIM-1 (green), and nuclei (blue). (a,c,e,g) are cells antibody secondary labeled and correspond to the negative controls.
Figure 3
Figure 3
Expression of AXL and TIM-1 receptors in PC3 (a) and Vero E6. (b) The infected and uninfected cells after 24 h were detected by flow cytometry. Analysis shows mean and SEM (Standard Error Medium) from independent experiments.
Figure 4
Figure 4
ZIKV replicates in PC3 cells. Viral kinetics was determined in PC3 (a,b) and Vero E6 (c,d) cells infected at an MOI = 1 at 0–120 h.p.i, and 0 h.p.i corresponds to viral input. Characterization of the replicative cycle was established by plaque-forming units (a,c) and quantification of viral genomes (b,d). Analysis shows mean and SEM from independent experiments.
Figure 5
Figure 5
Innate immune response to Zika virus in PC3 (a) and Vero E6 (b) cells were evaluated at 6, 72, 96, and 120 h.p.i by RT-qPCR. mRNA expression of β and antiviral proteins (OAS1, Viperin, and SOCS-1) in ZIKV-infected PC3 (a) and Vero E6 (b) were determined. Data are presented as the mean ± SEM from three independent experiments.
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