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. 2022 Dec 12;27(24):8809.
doi: 10.3390/molecules27248809.

Antitrypanosomal, Antitopoisomerase-I, and Cytotoxic Biological Evaluation of Some African Plants Belonging to Crassulaceae; Chemical Profiling of Extract Using UHPLC/QTOF-MS/MS

Mostafa M Hegazy  1 , Wael M Afifi  1   2 , Ahmed M Metwaly  1 , Mohamed M Radwan  3   4 , Muhamad Abd-Elraouf  1 , Ahmed B M Mehany  5 , Eman Ahmed  6   7 , Shymaa Enany  8 , Shahd Ezzeldin  6 , Adel E Ibrahim  9   10 , Sami El Deeb  9   11 , Ahmad E Mostafa  1
Affiliations

Antitrypanosomal, Antitopoisomerase-I, and Cytotoxic Biological Evaluation of Some African Plants Belonging to Crassulaceae; Chemical Profiling of Extract Using UHPLC/QTOF-MS/MS

Mostafa M Hegazy et al. Molecules. .

Abstract

In our continuous study for some African plants as a source for antitrypanosomally and cytotoxic active drugs, nine different plants belonging to the Crassulaceae family have been selected for the present study. Sedum sieboldii leaves extract showed an antitrypanosomal activity against Trypanosoma brucei with an IC50 value of 8.5 µg/mL. In addition, they have cytotoxic activities against (HCT-116), (HEPG-2) and (MCF-7), with IC50 values of 28.18 ± 0.24, 22.05 ± 0.66, and 26.47 ± 0.85 µg/mL, respectively. Furthermore, the extract displayed inhibition against Topoisomerase-1 with an IC50 value of 1.31 µg/mL. It showed the highest phenolics and flavonoids content among the other plants' extracts. In order to identify the secondary metabolites which may be responsible for such activities, profiling of the polar secondary metabolites of S. sieboldii extract via Ultra-Performance Liquid Chromatography coupled to High-Resolution QTOF-MS operated in negative and positive ionization modes, which revealed the presence of 46 metabolites, including flavonoids, phenolic acids, anthocyanidins, coumarin, and other metabolites.

Keywords: Crassulaceae; UHPLC/QTOF-MS; antitrypanosomal; cytotoxic; flavonoids; phenolics.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Compounds tentatively identified by UHPLC/QTOF–MS of S. sieboldii extract.
See this image and copyright information in PMC

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