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. 2022 Nov 28;11(12):1435.
doi: 10.3390/pathogens11121435.

Genetic and Antimicrobial Resistance Profiles of Mammary Pathogenic E. coli (MPEC) Isolates from Bovine Clinical Mastitis

Fernanda C Campos  1 , Ivana G Castilho  1 , Bruna F Rossi  1 , Érika C R Bonsaglia  1 , Stéfani T A Dantas  1 , Regiane C B Dias  1 , Ary Fernandes Júnior  1 , Rodrigo T Hernandes  1 , Carlos H Camargo  2 , Márcio G Ribeiro  3 , José C F Pantoja  3 , Hélio Langoni  3 , Vera L M Rall  1
Affiliations

Genetic and Antimicrobial Resistance Profiles of Mammary Pathogenic E. coli (MPEC) Isolates from Bovine Clinical Mastitis

Fernanda C Campos et al. Pathogens. .

Abstract

Mammary pathogenic E. coli (MPEC) is one of the main pathogens of environmental origin responsible for causing clinical mastitis worldwide. Even though E. coli are strongly associated with transient or persistent mastitis and the economic impacts of this disease, the virulence factors involved in the pathogenesis of MPEC remain unknown. Our aim was to characterize 110 MPEC isolates obtained from the milk of cows with clinical mastitis, regarding the virulence factor-encoding genes present, adherence patterns on HeLa cells, and antimicrobial resistance profile. The MPEC isolates were classified mainly in phylogroups A (50.9%) and B1 (38.2%). None of the isolates harbored genes used for diarrheagenic E. coli classification, but 26 (23.6%) and 4 (3.6%) isolates produced the aggregative or diffuse adherence pattern, respectively. Among the 22 genes investigated, encoding virulence factors associated with extraintestinal pathogenic E. coli pathogenesis, fimH (93.6%) was the most frequent, followed by traT (77.3%) and ompT (68.2%). Pulsed-field gel electrophoresis analysis revealed six pulse-types with isolates obtained over time, thus indicating persistent intramammary infections. The genes encoding beta-lactamases detected were as follows: blaTEM (35/31.8%); blaCTX-M-2/blaCTX-M-8 (2/1.8%); blaCTX-M-15 and blaCMY-2 (1/0.9%); five isolates were classified as extended spectrum beta-lactamase (ESBL) producers. As far as we know, papA, shf, ireA, sat and blaCTX-M-8 were detected for the first time in MPEC. In summary, the genetic profile of the MPEC studied was highly heterogeneous, making it impossible to establish a common genetic profile useful for molecular MPEC classification. Moreover, the detection of ESBL-producing isolates is a serious public health concern.

Keywords: ESBL; MPEC; intramammary infection; phylogroup; virulence.

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Conflict of interest statement

The authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript.

Figures

Figure 1
Figure 1
Dendrogram of PFGE patterns with >95% similarity coefficients for E. coli isolates obtained from the milk of cows with clinical mastitis. (*): 100% similarity between phylogroups A and unknown; (**), and between phylogroups A and C.
See this image and copyright information in PMC

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