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. 2022 Dec 19;11(24):3578.
doi: 10.3390/plants11243578.

First Report of Phaeoacremonium iranianum Causing Olive Twig and Branch Dieback

Elena Petrović  1 , Karolina Vrandečić  2 , Jasenka Ćosić  2 , Gabriella Kanižai Šarić  2 , Sara Godena  1
Affiliations

First Report of Phaeoacremonium iranianum Causing Olive Twig and Branch Dieback

Elena Petrović et al. Plants (Basel). .

Abstract

In an olive orchard on the western part of Istria, Croatia, twig and branch dieback was observed on several olive trees. In total, seven samples from symptomatic trees were collected. Samples were analyzed, and four fungal isolates showed morphological similarities to the species Phaeoacremonium. One isolate, chosen as a representative, was taken for molecular identification and pathogenicity tests. Based on the DNA sequence data of the ITS, TUB, and EF1α gene regions, the isolate was identified as P. iranianum. Pathogenicity tests were conducted on detached olive branches and olive trees in the greenhouse. To the best of our knowledge, this is the first report of twig and branch dieback on olive caused by Phaeoacremonium iranianum.

Keywords: Phaeoacremonium iranianum; dieback; olive.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
(a,b) Disease symptoms on olive branches in an orchard near Rovinj in Istria, Croatia, in the year 2021.
Figure 2
Figure 2
(a) Phaeoacremonium iranianum colony, on PDA, after two weeks in the dark at 25 °C. (b) P. iranianum colony, on PDA, after one month. (c) Micrographs of P. iranianum isolate under the microscope. Scale bar = 10 µm. (d) Hyaline, ovoid conidia.
Figure 3
Figure 3
(a–d) Disease symptoms on branches used in pathogenicity tests from March to July 2022.
See this image and copyright information in PMC

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