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Comment
. 2023 Jan;20(1):46-47.
doi: 10.1038/s41592-022-01702-0.

3D vascularized eye tissue models age-related macular degeneration

No authors listed
Comment

3D vascularized eye tissue models age-related macular degeneration

No authors listed. Nat Methods. 2023 Jan.
No abstract available

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References

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    1. Bhutto, I. & Lutty, G. Understanding age-related macular degeneration (AMD): relationships between the photoreceptor/retinal pigment epithelium/Bruch’s membrane/choriocapillaris complex. Mol. Aspects Med. 33, 295–317 (2012). This article reports physiological crosstalk between RPE, Bruch’s membrane and the choriocapillaris in AMD. - DOI
    1. Baltazar, T. et al. Three dimensional bioprinting of a vascularized and perfusable skin graft using human keratinocytes, fibroblasts, pericytes, and endothelial cells. Tissue Eng. A 26, 227–238 (2020). This article reports a detailed protocol for how to develop a 3D vasculature using bioprinting. - DOI
    1. Orlova, V. V. et al. Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells. Nat. Protoc. 9, 1514–1531 (2014). This article reports a detailed step-by-step protocol to differentiate endothelial cells and pericytes from iPSCs. - DOI
    1. Sharma, R. et al. Clinical-grade stem cell-derived retinal pigment epithelium patch rescues retinal degeneration in rodents and pigs. Sci. Transl. Med. 11, eaat5580 (2019). This article reports a detailed protocol to differentiate RPE cells and pericytes from iPSCs. - DOI

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