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. 2023 May;25(5):1007-1021.
doi: 10.1111/1462-2920.16326. Epub 2023 Jan 21.

Growth dynamics and transcriptional responses of a Red Sea Prochlorococcus strain to varying temperatures

Abbrar Labban  1   2 , Ahmed A Shibl  3   4 , Maria Ll Calleja  5 , Pei-Ying Hong  2 , Xosé Anxelu G Morán  1   6
Affiliations

Growth dynamics and transcriptional responses of a Red Sea Prochlorococcus strain to varying temperatures

Abbrar Labban et al. Environ Microbiol. 2023 May.

Abstract

Prochlorococcus play a crucial role in the ocean's biogeochemical cycling, but it remains controversial how they will respond to global warming. Here we assessed the response to temperature (22-30°C) of the growth dynamics and gene expression profiles of a Red Sea Prochlorococcus strain (RSP50) in a non-axenic culture. Both the specific growth rate (0.55-0.80 day-1 ) and cell size (0.04-0.07 μm3 ) of Prochlorococcus increased significantly with temperature. The primary production released extracellularly ranged from 20% to 34%, with humic-like fluorescent compounds increasing up to fivefold as Prochlorococcus reached its maximum abundance. At 30°C, genes involved in carbon fixation such as CsoS2 and CsoS3 and photosynthetic electron transport including PTOX were downregulated, suggesting a cellular homeostasis and energy saving mechanism response. In contrast, PTOX was found upregulated at 22°C and 24°C. Similar results were found for transaldolase, related to carbon metabolism, and citrate synthase, an important enzyme in the TCA cycle. Our data suggest that in spite of the currently warm temperatures of the Red Sea, Prochlorococcus can modulate its gene expression profiles to permit growth at temperatures lower than its optimum temperature (28°C) but is unable to cope with temperatures exceeding 30°C.

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References

REFERENCES

    1. Agusti, S., Lubián, L.M., Moreno-Ostos, E., Estrada, M. & Duarte, C.M. (2019) Projected changes in photosynthetic picoplankton in a warmer subtropical ocean. Frontiers in Marine Science, 506.
    1. Al-Otaibi, N., Huete-Stauffer, T.M., Calleja, M.L., Irigoien, X. & Moran, X.A.G. (2020) Seasonal variability and vertical distribution of autotrophic and heterotrophic picoplankton in the Central Red Sea. PeerJ, 8, e8612.
    1. Atkinson, D. & Sibly, R.M. (1997) Why are organisms usually bigger in colder environments? Making sense of a life history puzzle. Trends in Ecology & Evolution, 12, 235-239.
    1. Bailey, S., Melis, A., Mackey, K.R., Cardol, P., Finazzi, G., van Dijken, G. et al. (2008) Alternative photosynthetic electron flow to oxygen in marine Synechococcus. Biochimica et Biophysica Acta, 1777(3), 269-276.
    1. Bertilsson, S., Berglund, O., Pullin, M.J. & Chisholm, S.W. (2005) Release of dissolved organic matter by Prochlorococcus. Vie et Milieu, 55, 225-232.

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