Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct-Dec;13(4):296-300.
doi: 10.4103/japtr.japtr_475_22. Epub 2022 Oct 10.

Ethanolic extract of Lannea coromandelica stem bark: Histopathology and infiltration of inflammatory cells in the rat's gastric

Achmad Ramadhan  1 , Hastuti Herman  1 , Sutrisnawati Sutrisnawati  1
Affiliations

Ethanolic extract of Lannea coromandelica stem bark: Histopathology and infiltration of inflammatory cells in the rat's gastric

Achmad Ramadhan et al. J Adv Pharm Technol Res. 2022 Oct-Dec.

Abstract

This study determined the efficacy of extract of the stem bark of Lannea coromandelica (ESBLc) on histopathology and inflammatory cell infiltration in the gastric of rats induced by mefenamic acid. We grouped 20 rats (Rattus norvegicus) into 5; Group 1 (positive control, mefenamic acid + sucralfate suspension), Group 2 (negative control, mefenamic acid), Group 3 (mefenamic acid + ESBLc 1575 mg/kg), Group 4 (mefenamic acid + ESBLc 3150 mg/kg), and Group 5 (mefenamic acid + ESBLc 3600 mg/kg). The dose of mefenamic acid used was 23.25 mg/kg, given orally for 7 days. Gastric histopathological observations were carried out qualitatively, and inflammatory cell infiltration was analyzed quantitatively by one-ay ANOVA. The qualitative and quantitative analysis results showed that ESBLc had efficacy in restoring damaged gastric tissue of rats; statistically, 3150 mg/kg and 6300 mg/kg effectively reduced inflammatory cell infiltration. ESBLc recovered the function of gastric organs of Rattus norvegicus L. induced by mefenamic acid, including improved mucosa and reduced inflammatory cell infiltration in the gastric. The doses of ESBLc, which effectively reduced inflammatory cell infiltrations, were 3150 mg/kg and 6300 mg/kg BW.

Keywords: Ethanolic extract; Lannea coromandelica; gastric; histopathology; inflammatory.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Histopathology of white rat's gastric. (a) (Group 1 positive control) reduced epithelial cell desquamation (blue arrow) and inflammatory cell infiltration (yellow arrow). (b) (Group 2 negative control) found epithelial cells desquamation and inflammatory cell infiltration. (c) (Group-3) Epithelial cell desquamation and inflammatory cell infiltration were still visible. (d) (Group-4) Epithelial cell desquamation was decreasing. (e) (Group-5) Normal epithelial cell desquamation, very less inflammatory cell infiltration (×100)
Figure 2
Figure 2
Histopathology of white rat's gastric. (a) (Negative control) moderately severe mucosal erosion (black arrow). (b) (Positive control) found mucosal erosion. (c) (Group-3) Mucosal erosions are visible. (d) (Group-4) mucosal erosion was decreasing. (e) (Group-5) mucosal cell erosion began to appear normal (×100)
See this image and copyright information in PMC

References

    1. Parolini M. Toxicity of the Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) acetylsalicylic acid, paracetamol, diclofenac, ibuprofen and naproxen towards freshwater invertebrates: A review. Sci Total Environ. 2020;740:140043. - PubMed
    1. Ayoub R, Jarrar Q, Ali D, Moshawih S, Jarrar Y, Hakim M, et al. Synthesis of novel esters of mefenamic acid with pronounced anti-nociceptive effects and a proposed activity on GABA, opioid and glutamate receptors. Eur J Pharm Sci. 2021;163:105865. - PubMed
    1. Fenton C, Kang C. Other options usually better than benzodiazepines in alleviating dyspnoea, sleeping problems and anxiety in COPD patients. Drugs Ther Perspect. 2021;37:19–24.
    1. Gilbertson J, Quintanar-Solares M, Liland F, Niermeyer S. High-level disinfection of re-usable neonatal resuscitation equipment through boiling and steaming. J Hosp Infect. 2020;106:721–5. - PubMed
    1. Shaydakov ME, Ting W, Sadek M, Aziz F, Diaz JA, Raffetto JD, et al. Review of the current evidence for topical treatment for venous leg ulcers. J Vasc Surg Venous Lymphat Disord. 2022;10:241–7.e15. - PubMed