Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan;9(1):282-289.
doi: 10.1002/vms3.1059. Epub 2022 Dec 26.

The wound healing effect of collagen/adipose-derived stem cells (ADSCs) hydrogel: In vivo study

Kamyar Abbasi  1 , Sara Tavakolizadeh  1 , Alireza Hadi  1 , Maryam Hosseini  2 , Reza Sayyad Soufdoost  3 , Artak Heboyan  4 , Mostafa Alam  5 , Sadaf Fani-Hanifeh  6
Affiliations

The wound healing effect of collagen/adipose-derived stem cells (ADSCs) hydrogel: In vivo study

Kamyar Abbasi et al. Vet Med Sci. 2023 Jan.

Abstract

Background: The complex wound healing process involves activating and synchronizing intracellular, intercellular, and extracellular components. Adipose tissue is attracting attention to promote wound healing. Within subcutaneous adipose tissue, stromal vascular cells and their subsets release growth factors and cytokines critical for neovascularization and wound repair.

Objectives: This study evaluated human placental collagen/adipose-derived stem cells (ADSCs) hydrogel for wound healing in rats.

Methods: In this study, ADSCs were harvested, cultured, and mixed with placental collagen. Twelve rats were used, and their backs were excised three times each. Group one received collagen/ADSCs, group two collagen, and group three non-filled (control) excisions. The healing processes were assessed by histological analysis, taking photographs, and calculating the percentage of wound contraction in mentioned times.

Results: Histopathological analysis revealed that the content of fibroblasts, follicles of the hair, and angiogenesis in group one was significantly more than in other groups. Group one had a significant result compared with the collagen and control groups. In group one, significant wound healing and wound contraction were observed with 52% and 80% wound contraction at 7 and 14 days, respectively.

Conclusion: Collagen/ADSCs can be considered a suitable candidate hydrogel in wound healing with a high potential for enhancing wound repairing.

Keywords: ADSCs; Wound healing; collagen; hydrogel; placenta.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests

Figures

FIGURE 1
FIGURE 1
Flow Cytometry graphs. The low expression of CD 34, 45, and the high expression of CD 90 and 105 markers of mesenchymal stem cells
FIGURE 2
FIGURE 2
Macroscopic evaluation of the treated and untreated groups
FIGURE 3
FIGURE 3
H and E staining evaluation of the collagen/ADSCs group (×40 magnification)
FIGURE 4
FIGURE 4
H and E staining evaluation of the collagen group at 7 and 14 days (×40 magnification)
FIGURE 5
FIGURE 5
H and E staining evaluation of the control group at 7 and 14 days (×40 magnification)
See this image and copyright information in PMC

References

    1. An, Y. , Lin, S. , Tan, X. , Zhu, S. , Nie, F. , Zhen, Y. , Gu, L. , Zhang, C. , Wang, B. , Wei, W. , Li, D. , & Wu, J. (2021). Exosomes from adipose‐derived stem cells and application to skin wound healing. Cell Proliferation, 54(3), e12993. - PMC - PubMed
    1. Barzegar, P. E. F. , Ranjbar, R. , Yazdanian, M. , Tahmasebi, E. , Alam, M. , Abbasi, K. , Tebyaniyan, H. , & Esmaeili Fard Barzegar, K. (2022). The current natural/chemical materials and innovative technologies in periodontal diseases therapy and regeneration: A narrative review. Materials Today Communications, 32, 104099.
    1. Beahm, E. K. , Walton, R. L. , & Patrick, C. W. (2003). Progress in adipose tissue construct development. Clinics in Plastic Surgery, 30(4), 547–558. - PubMed
    1. Cigna, E. , Tarallo, M. , Bistoni, G. , Anniboletti, T. , Trignano, E. , Tortorelli, G. , & Scuderi, N. (2009). Evaluation of polyurethane dressing with ibuprofen in the management of split‐thickness skin graft donor sites. In Vivo (Athens, Greece), 23(6), 983–986. - PubMed
    1. El‐Ashram, S. , El‐Samad, L. M. , Basha, A. A. , & El Wakil, A. (2021). Naturally‐derived targeted therapy for wound healing: Beyond classical strategies. Pharmacological Research, 170, 105749. - PubMed

LinkOut - more resources