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. 2022 Dec 26;108(2):356-358.
doi: 10.4269/ajtmh.22-0478. Print 2023 Feb 1.

Long-term Persistence of Opisthorchis viverrini Antigen in Urine: A Prospective Study in Northeast Thailand

Chanika Worasith  1   2 , Phattharaphon Wongphutorn  2   3 , Kulthida Y Kopolrat  4 , Chutima Homwong  2 , Anchalee Techasen  2   5 , Raynoo Thanan  6 , Chompunoot Wangboon  7 , Chatanun Eamudomkarn  1   2 , Jiraporn Sithithaworn  5 , Thomas Crellen  8   9 , Paiboon Sithithaworn  1   2
Affiliations

Long-term Persistence of Opisthorchis viverrini Antigen in Urine: A Prospective Study in Northeast Thailand

Chanika Worasith et al. Am J Trop Med Hyg. .

Abstract

Antigen detected in urine for the diagnosis of opisthorchiasis has a low daily variation; however, the longer term variability in antigen concentrations is unknown. In this study, we prospectively monitored Opisthorchis viverrini antigen concentrations for 30 consecutive days and at subsequent monthly intervals in a cohort of opisthorchiasis-positive individuals. On the basis of the monoclonal antibody-based ELISA, the profiles of antigen-positive rate and antigen concentration exhibited no significant change over 30 days with a mean proportion positive of 87.1% (range 73.7%-100%), and the average antigen concentration was 29.7 ± 2.2 ng/mL (mean ± SE). The urine antigen concentration at baseline was similar to the subsequent measurements at 2, 4, 6, and 10 months in the follow-up study (P > 0.05). The consistency and low daily and long-term fluctuation of O. viverrini antigen in urine demonstrates the reliability of urine assay for diagnosis of opisthorchiasis.

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Figures

Figure 1.
Figure 1.
The profiles of positive detection rate for opisthorchiasis and urine antigen concentration (nanograms per milliliter) over 30 days. The percentage urine antigen positive in each day (x-axis) correspond to the right y-axis (%) with an overall prevalence of 87.1 (black-dotted line). Data shown (dots) are urine antigen concentrations for individual participants, which correspond to the left y-axis (log-transformed antigen concentration, nanograms per milliliter). The red-dotted line indicates the cutoff value for a positive urine antigen assay (> 19.4 ng/mL). Urine samples were obtained from 35 to 42 participants per day.
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References

    1. Sithithaworn P Andrews RH Nguyen VD Wongsaroj T Sinuon M Odermatt P Nawa Y Liang S Brindley PJ Sripa B , 2012. The current status of opisthorchiasis and clonorchiasis in the Mekong Basin. Parasitol Int 61: 10–16. - PMC - PubMed
    1. Sripa B Suwannatrai AT Sayasone S Do DT Khieu V Yang Y , 2021. Current status of human liver fluke infections in the Greater Mekong Subregion. Acta Trop 224: 106133. - PubMed
    1. Crellen T Sithithaworn P Pitaksakulrat O Khuntikeo N Medley GF Hollingsworth TD , 2021. Towards evidence-based control of Opisthorchis viverrini. Trends Parasitol 37: 370–380. - PubMed
    1. Khuntikeo N et al. 2018. Current perspectives on opisthorchiasis control and cholangiocarcinoma detection in Southeast Asia. Front Med (Lausanne) 5: 117. - PMC - PubMed
    1. Sayasone S Utzinger J Akkhavong K Odermatt P , 2015. Repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a cross-sectional survey in southern Lao People’s Democratic Republic. Acta Trop 141: 315–321. - PubMed