Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec 26;12(1):22348.
doi: 10.1038/s41598-022-26909-1.

Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique

Putri Kusuma Astuti  1   2 , Daniela Elena Ilie  3 , Dinu Gavojdian  4 , George Wanjala  1   2 , Bouabid Badaoui  5 , Husein Ohran  6 , Eva Pasic-Juhas  6 , Zoltán Bagi  1 , András Jávor  1 , Szilvia Kusza  7
Affiliations

Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique

Putri Kusuma Astuti et al. Sci Rep. .

Abstract

A study on 51 SNPs belonging to 29 genes related to heat stress was carried out in 720 sheep from 17 different breeds adapted to different climates from Hungary, Bosnia and Herzegovina, Morocco and Romania, using Kompetitive Allele-Specific Polymerase Chain Reaction. Genotype frequency and the Hardy-Weinberg equilibrium were calculated, followed by a clustering using the Principal Component Analysis. We analyzed the polymorphisms in the following genes analyzed: HSPA12A, HSP90AA1, IL33, DIO2, BTNL2, CSN2, ABCG1, CSN1S1, GHR, HSPA8, STAT3, and HCRT. We emphasized on HSPA12A and HSPA8 genes as they were successfully genotyped in all studied flocks in which genotype frequency patterns were identified. Contrary to previous findings, the A allele for HSPA8 SNP was not observed in the heat tolerant breeds, being found exclusively in cold-tolerant breeds. The principal component analysis could not clearly differentiate the breeds, while plot concentration was slightly varied among the three groups, with HSP90AA1 and IL33 SNPs' loading values significantly contributing to PC1 and PC2. We confirmed previous works that the HSPA12A, HSPA8, HSP90AA1 and IL33 SNPs are potential candidate markers for thermotolerance adaptation in sheep. This research contributes to the genetic variability of SNPs for thermotolerance adaptability in sheep.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Score biplot of Principal Component Analysis of the 17 SNPs and 601 animals. (a) Individuals of different breeds are differently colored; (AWAS Hungarian Awassi, BG Béni Guil, DM D’Man, HUMER Hungarian Merino, HURAC Hungarian Racka, HUTSI Hungarian Tsigai, IDF Ile de France, KAR Botosani Karakul, PRA Pramenka, RORAC Romanian Racka, ROTSI Romanian Tsigai, SAR Sardi, SUF Suffolk, TET Babolna Tetra, TIM Timahdite, TRANSMER Transylvanian Merino and TUR Turcana). (b) Breeds grouped by climatic characteristics; COLD: cold-tolerant breeds (Suffolk, Babolna Tetra, Ile de France, Hungarian Tsigai, Hungarian Racka, Hungarian Merino, Pramenka, Romanian Racka and Turcana), HOT: heat tolerant breeds originated from Morocco (Béni Guil, D’Man, Timahdite and Sardi), HOT EU: Heat tolerant breeds reared in Europe (Hungarian Awassi, Botosani Karakul, Transylvanian Merino and Romanian Tsigai).
Figure 2
Figure 2
Loading biplot of principal component analysis of the 17 SNPs for 17 sheep breeds.
See this image and copyright information in PMC

References

    1. IPCC. Summary for Policymakers. In (Masson-Delmotte, V., P. Zhai, H.-O. Pörtner, D. Roberts, J. Skea, P.R. Shukla, A. Pirani, W. Moufouma-Okia, C. Péan, R. Pidcock, S. Connors, J.B.R. Matthews, Y. Chen, X. Zhou, M.I. Gomis, E. Lonnoy, T. Maycock, M. Tignor, and T. Waterfield, eds) Global Warming of 1.5°C. An IPCC Special Report on the impacts of global warming of 1.5°C above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty. In Press (2018).
    1. Malhi Y, Franklin J, Seddon N, Solan M, Turner MG, Field CB, Knowlton N. Climate change and ecosystems: Threats, opportunities and solutions. Philos. Trans. R. Soc. B Biol. Sci. 2020;375(1794):20190104. doi: 10.1098/rstb.2019.0104. - DOI - PMC - PubMed
    1. McElwee P. Climate change and biodiversity loss. Curr. Hist. 2021;120(829):295–300. doi: 10.1525/curh.2021.120.829.295. - DOI
    1. Dickinson MG, Orme CDL, Suttle KB, Mace GM. Separating sensitivity from exposure in assessing extinction risk from climate change. Sci. Rep. 2015;4(1):6898. doi: 10.1038/srep06898. - DOI - PMC - PubMed
    1. UNFCCC (United Nations Framework Convention on Climate Change). Global Warming Potentials http://unfccc.int/ghg_data/items/3825.php (2014).

Publication types